生化實驗英翻中

原文：

We have demonstrated on-chip RNA isolation in picoliter droplets, reverse transcription within the individual droplets, and subsequent real-time PCR with fluorescence detection of amplification in each interrogated droplet. The system employed a method of sample partitioning into monodisperse picoliter droplets emulsified in oil to generate isolated chemical reactors for highsensitivity nucleic acid detection. The method required 23 cycles for single-copy real-time reverse transcription from RNA, amplification, and detection on-chip using Taqman-based probes. The results show the method is well-suited to quantitative PCR applications, given the observability of Poisson statistics in the picodroplet-discretized sample. Applying digital microfluidics to real-time RT-PCR combines the advantages of on-chip processing of picoliter reactors with single-copy detection.