方法一，用現成的商業產品，比方: Romer Labs® 的AgraQuant® Melamine ELISA Test Kit
http://www.romerlabs.com/pdts_kits.html
以下摘自此網頁：
AgraQuant® Melamine ELISA Test Kit
The AgraQuant® Melamine Test Kit by Romer Labs® is a direct competitive enzyme-linked immunosorbent assay (ELISA). It has been developed for the analysis of melamine in wheat gluten and pet food.
AgraQuant® Melamine Test Kit has quantitation ranges of 10-250ppm for wheat gluten, 2-50ppm for moist pet food, and 4-100ppm for dried pet food. Each kit comes complete with melamine standards, antibody-coated microwells, enzyme conjugate, substrate and stop solution, etc.
The AgraQuant® Melamine Kit is accurate; the results are comparable with published HPLC methods. The method is reproducible; consistent results have been obtained in intra- and inter-laboratory settings. The AgraQuant® Melamine Kit is very fast and can be run with 60 minutes total incubation time. Simple methanol/water sample extraction and no clean-up steps required make the test very easy to use. The 96 breakaway microwell format minimizes waste and maximizes value and makes the test very cost-effective.
(本人與此公司毫無關係)

方法二，美國FDA三聚氰胺檢測的方法(依據Wikipedia的說法)
高效液相色譜法測定三聚氰胺， Ammeline ， Ammelide ，和氰尿酸污染的麥麩和濃縮大米蛋白
羅伯特史密斯 ，吉米幹特和瓊Nandrea 。 美國FDA實驗室， Lenexa ，美國堪薩斯

以pH值為2.5 ， 50毫米的磷酸鹽緩衝液提取小麥麵筋或大米蛋白濃縮物，在60度攝氏下大力攪拌四個小時。將該提取物注射到一個組合在兩欄系列（短強陽離子交換柱和C18柱）以pH值為2.5 ， 50毫米的磷酸鹽緩衝液洗脫 。三聚氰胺， ammeline和氰尿酸中的原始材料，從噪音水平中估計檢測限（ LOD值）和定量限（ LOQ ） ，大約為100 μg/mL和400 μg/mL。  Ammelide的LOD和LOQ估計分別為10 μg/mL和40 μg/mL。 穗回收率分別為98 ％ 到110 ％ 。 這種方法的有用定量範圍為0.1 ％至5 ％的三聚氰胺和氰尿酸，從400 ppm到10000 ppm的ammeline和從50 ppm到10000 ppm的ammelide的小麥麵筋或大米蛋白。

本人化學程度有限，原文摘譯自:
http://acs.confex.com/acs/mwrm07/techprogram/P51682.HTM
HPLC Determination of Melamine, Ammeline, Ammelide, and Cyanuric Acid Contamination in Wheat Gluten and Rice Protein Concentrate
Robert Smith, Jimmie Weigand, and Joan Nandrea. US FDA Laboratory, Lenexa, KS

Wheat gluten or rice protein concentrate was extracted with pH 2.5, 50 mM phosphate buffer by stirring vigorously for four hours at 60°C. The extract was injected onto a combination of two columns in series (a short strong cation exchange column and a C18 column) and eluted using a 50 mM phosphate pH 2.5 buffer. The limit of detection (LOD) and limit of quantification (LOQ) were estimated from the noise level to be approximately 100 µg/mL and 400µg/mL respectively for melamine, ammeline, and cyanuric acid in the original material. Ammelide LOD and LOQ were estimated at 10µg/mL and 40µg/mL respectively. Spike recoveries were 98% to110%. This method is useful for quantitation in the range of 0.1% to 5% for melamine and cyanuric acid, and from 400 ppm to 10,000 ppm for ammeline and from 50 ppm to 10,000 ppm for ammelide in the wheat gluten or rice protein.

方法三(開發中)，2008 Intel 國際科學工程展(ISEF)獎得主，父母為斯里蘭卡人的美國高中生Natalie Saranga Omattage提出的檢測方法:
http://www.youtube.com/watch?v=8Adjzxp7BP8