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文章數：209 |
 永心鳳茶海鮮表現如何？》公益路美食2026最新版｜10家必吃大評比 |
| 心情隨筆｜心靈 2026/04/21 08:07:33 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人，臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」，從精緻西餐到創意火鍋，從日式丼飯到義式早午餐，每走幾步，就會有完全不同的特色料理餐廳。 這次我特別花了一整個月，實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店，也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準，整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你，找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型，從高質感牛排館到巷弄系早午餐，每一間都有自己獨特的風格。為了讓整體比較更客觀，我依照以下四大面向進行評比，並搭配實際用餐體驗來打分。
整體而言，我希望這份評比不只是「哪家好吃」，而是幫你在不同情境下（約會、家庭聚餐、朋友小聚、商業午餐）都能快速找到合適的選擇。畢竟，美食不只是味覺的滿足，更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段，從火鍋、燒肉到中式料理與早午餐，每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單，橫跨平價、創意、高級各路風格。
一頭牛日式燒肉｜炭香濃郁的和牛饗宴，約會聚餐首選
走在公益路上，很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面，搭配昏黃燈光與暖色調的內裝，讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大，但桌距規劃得宜，每桌皆設有獨立排煙設備，烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂，絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網，讓人完全不用分心。整場用餐過程就像一場表演，從視覺、嗅覺到味覺都被滿足。 綜合評分
地址：408臺中市南屯區公益路二段162號電話：04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」，更像是一場五感盛宴。從進門那一刻到最後一道甜點，都能感受到他們對細節的用心。 TANG Zhan 湯棧｜文青系火鍋代表，麻香湯底與視覺美感並重
在公益路這條美食戰線上，TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址：408臺中市南屯區公益路二段248號電話：04-22580617 官網：https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度，又結合現代風格與細節服務，讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店，湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店｜明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂，那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主，陽光灑進室內，讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧，建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味，選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口，米芯保留微Q口感；而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香，口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹，無論是一個人帶電腦工作、或朋友聚餐，都能找到舒服角落。餐點上桌速度穩定，服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」，很適合想遠離忙碌日常的人。 綜合評分
地址：40861臺中市南屯區公益路二段18號電話：04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇，而是以「剛剛好」的份量與風味，陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店，NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物｜平價卻用心的湯頭系火鍋，家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上，加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐，但靠著實在的湯頭與親切的服務，默默吸引許多回頭客。每到用餐時間，總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底，湯頭清澈卻不單薄，越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向，桌距寬敞、座位舒適，帶小孩來也不覺擁擠。店員態度親切，補湯、收盤都很勤快，給人一種「被照顧著」的安心感。 綜合評分
地址：403臺中市西區公益路288號電話：0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤，而是用最簡單的湯頭與新鮮食材，傳遞出家常卻不平凡的溫度。 印月餐廳｜中式料理的藝術演繹，宴客與家庭聚會首選
說到臺中公益路的中式料理代表，印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名，把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設，每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏，都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法，讓人感受到「被款待」的尊榮感。 綜合評分
地址：408臺中市南屯區公益路二段818號電話：0422511155 小結語印月餐廳是一間「不只吃飯，更像品味生活」的地方。 KoDō 和牛燒肉｜極致職人精神，專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗，一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉，以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址：403臺中市西區公益路260號電話：0423220312 官網：https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳，而是一場體驗。 永心鳳茶｜在茶香裡用餐的優雅時光，臺味早午餐的新詮釋
走進 永心鳳茶公益店，彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤，無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」，都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和，對茶品介紹詳盡。上餐節奏剛好，不急不徐。 綜合評分
地址：40360臺中市西區公益路68號三樓(勤美誠品)電話：0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓｜老饕級江浙功夫菜，穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主，兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址：408臺中市南屯區公益路二段95號電話：0423202322 官網：https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司｜低調奢華的無菜單日料，職人手藝詮釋旬味極致
在熱鬧的公益路上，一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase（無菜單料理） 形式，每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘，節奏緩慢但沉穩。 綜合評分
地址：408臺中市南屯區公益路二段25號電話：0423206368 官網：https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂｜人氣爆棚的和牛燒肉聖地，肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳，茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐，價格約落在 $700–$1000 間，份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快，整場用餐流程流暢無壓力。 綜合評分
地址：403臺中市西區公益路268號電話：0423281167 官網：https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質＋輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後，臺中公益路不只是一條美食街，而是一段生活風景線。 有的餐廳講究細膩與儀式感，像 一頭牛日式燒肉 與 一笈壽司，讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心，像 加分昆布鍋物、永心鳳茶，讓人明白吃飯不只是為了飽足，而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店，則用穩定的品質與熱絡的氛圍，成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店，構成了公益路最動人的縮影 有華麗的，也有溫柔的；有傳統的，也有創新的。 每一家都在自己的風格裡發光，讓人吃到的不只是料理，而是一種生活的溫度與節奏。 對我而言，這不僅是一場美食旅程，更是一趟關於「臺中味道」的回憶之旅。 FAQ：關於臺中公益路美食常見問題Q1：公益路哪一區的餐廳最集中？ Q2：需要提前訂位嗎？ 最後的話若要用一句話形容這趟美食之旅，我會說： 一笈壽司好吃嗎？ 如果你也和我一樣喜歡用味蕾探索一座城市，那就把這篇公益路美食攻略收藏起來吧。三希樓公司聚餐適合嗎？ 無論是約會、慶生、家庭聚餐，或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。NINI 尼尼臺中店CP 值高嗎？ 下一餐，不妨從這10家開始。加分100%浜中特選昆布鍋物有什麼推薦搭配？ 打開手機、約上朋友，讓公益路成為你生活裡最容易抵達的小確幸。加分100%浜中特選昆布鍋物口味偏臺式還是日式？ 如果你有私心愛店，也歡迎留言分享，加分100%浜中特選昆布鍋物春酒活動適合在這裡辦嗎？ 你的推薦，可能讓我下一趟美食旅程變得更精彩。KoDō 和牛燒肉小資族值得嗎？ Scientists have identified a new hybrid brain cell, sharing attributes of neurons and astrocytes. This discovery could settle longstanding debates in neuroscience about the role of astrocytes in synaptic transmission. Researchers have discovered a new hybrid brain cell, bridging the gap between neurons and astrocytes. This cell can release neurotransmitters and may influence conditions like epilepsy and memory consolidation, offering promising paths for neuroscientific research and potential treatments. Neuroscience is in great upheaval. The two major families of cells that make up the brain, neurons and glial cells, secretly hid a hybrid cell, halfway between these two categories. For as long as Neuroscience has existed, it has been recognized that the brain works primarily thanks to the neurons and their ability to rapidly elaborate and transmit information through their networks. To support them in this task, glial cells perform a series of structural, energetic, and immune functions, as well as stabilize physiological constants. Some of these glial cells, known as astrocytes, intimately surround synapses, the points of contact where neurotransmitters are released to transmit information between neurons. This is why neuroscientists have long suggested that astrocytes may have an active role in synaptic transmission and participate in information processing. However, the studies conducted to date to demonstrate this have suffered from conflicting results and have not reached a definitive scientific consensus yet. By identifying a new cell type with the characteristics of an astrocyte and expressing the molecular machinery necessary for synaptic transmission, neuroscientists from the Department of Basic Neurosciences of the Faculty of Biology and Medicine of the University of Lausanne (UNIL) and the Wyss Center for Bio and Neuroengineering in Geneva put an end to years of controversy. The Key to the Puzzle To confirm or refute the hypothesis that astrocytes, like neurons, are able to release neurotransmitters, researchers first scrutinized the molecular content of astrocytes using modern molecular biology approaches. Their goal was to find traces of the machinery necessary for the rapid secretion of glutamate, the main neurotransmitter used by neurons. “The precision allowed by single-cell transcriptomics approaches enabled us to demonstrate the presence in cells with astrocytic profile of transcripts of the vesicular proteins, VGLUT, in charge of filling neuronal vesicles specific for glutamate release. These transcripts were found in cells from mice, and are apparently preserved in human cells. We also identified other specialized proteins in these cells, which are essential for the function of glutamatergic vesicles and their capacity to communicate rapidly with other cells,” says Ludovic Telley, Assistant professor at UNIL, co-director of the study. New Functional Cells Next, neuroscientists tried to find out if these hybrid cells were functional, that is, able to actually release glutamate with a speed comparable to that of synaptic transmission. To do this, the research team used an advanced imaging technique that could visualize glutamate released by vesicles in brain tissues and in living mice. “We have identified a subgroup of astrocytes responding to selective stimulations with rapid glutamate release, which occurred in spatially delimited areas of these cells reminiscent of synapses,” says Andrea Volterra, honorary professor at UNIL and visiting faculty at the Wyss Center, co-director of the study. In addition, this glutamate release exerts an influence on synaptic transmission and regulates neuronal circuits. The research team was able to demonstrate this by suppressing the expression of VGLUT by the hybrid cells. “They are cells that modulate neuronal activity, they control the level of communication and excitation of the neurons,” says Roberta de Ceglia, first author of the study and senior researcher at UNIL. And without this functional machinery, the study shows that long-term potentiation, a neural process involved in the mechanisms of memorization, is impaired and that the memory of mice is impacted. Links With Brain Pathologies The implications of this discovery extend to brain disorders. By specifically disrupting glutamatergic astrocytes, the research team demonstrated effects on memory consolidation, but also observed links with pathologies such as epilepsy, whose seizures were exacerbated. Finally, the study shows that glutamatergic astrocytes also have a role in the regulation of brain circuits involved in movement control and could offer therapeutic targets for Parkinson’s disease. “In between neurons and astrocytes, we now have a new kind of cell at hand. Its discovery opens up immense research prospects. Our next studies will explore the potential protective role of this type of cell against memory impairment in Alzheimer’s disease, as well as its role in other regions and pathologies than those explored here,” projects Andrea Volterra. Reference: “Specialized astrocytes mediate glutamatergic gliotransmission in the CNS” by Roberta de Ceglia, Ada Ledonne, David Gregory Litvin, Barbara Lykke Lind, Giovanni Carriero, Emanuele Claudio Latagliata, Erika Bindocci, Maria Amalia Di Castro, Iaroslav Savtchouk, Ilaria Vitali, Anurag Ranjak, Mauro Congiu, Tara Canonica, William Wisden, Kenneth Harris, Manuel Mameli, Nicola Mercuri, Ludovic Telley and Andrea Volterra, 6 September 2023, Nature. DOI: 10.1038/s41586-023-06502-w Koala in the Wild. Credit: A. Gillett Koala retrovirus (KoRV) is an active, inherited virus that disrupts gene function and is linked to high cancer rates in koalas. Tumor tissues show increased KoRV activity, and specific gene insertions may even be heritable, raising serious conservation concerns. The koala retrovirus (KoRV) is a virus that, like other retroviruses such as HIV, inserts itself into the DNA of an infected cell. At some point in the past 50,000 years, KoRV has infected the egg or sperm cells of koalas, leading to offspring that carry the retrovirus in every cell in their body. The entire koala population of Queensland and New South Wales in Australia now carry copies of KoRV in their genome. All animals, including humans, have gone through similar “germ line” infections by retroviruses at some point in their evolutionary history and contain many ancient retroviruses in their genomes. These retroviruses have, over millions of years, mutated into degraded, inactive forms that are no longer harmful to the host. Since in most animal species this process occurred millions of years ago, the immediate health effects on the host at that time are unknown but it has been suspected for some time that the invasion of a genome by a retrovirus may have considerable detrimental health effects. The koala is at a very early stage of this process when the retrovirus is still active and these health effects can be studied. High Cancer Rates Linked to KoRV Activity Since retroviruses can cause cancer, it was thought that there is a link between KoRV and the high frequency of lymphoma, leukemia, and other cancers in koalas from northern Australia. To investigate this link, scientists at the Leibniz-IZW sequenced DNA from wild koalas suffering from cancer. This allowed them to accurately detect the number of copies of KoRV in the koala genomes and identify the precise locations where the retrovirus had inserted its DNA. By comparing this information between healthy and tumor tissues in single koalas, and by comparing insertion sites between koala individuals, they found multiple links between KoRV and genes known to be involved in the kind of cancers to which koalas are prone. “Each koala carries around 80 – 100 inherited copies of KoRV in its genome. The genomic locations of most of these are not shared between koalas, indicating a rapid expansion and accumulation of KoRV copies in the population. Each time a retrovirus copies and re-inserts itself into the genome, it causes a mutation, potentially disrupting gene expression, which could be detrimental to the host,” says Prof Alex Greenwood, Head of Department of Wildlife Diseases at the Leibniz-IZW. This means that by frequently copying itself to new locations in the genome, KoRV is currently conferring a high mutational load on the koala population. Tumor tissues contain many new copies of KoRV, indicating that KoRV is more active in tumor cells. These copies generally were located close to genes associated with cancer. New KoRV insertions in tumor tissues affected the expression of genes in their vicinity. Such changes in gene expression associated with cancer can cause increased cell growth and proliferation, which leads to tumors. Although other factors may also contribute to cancer in koalas, the mutational burden from KoRV likely increases the frequency of cells becoming cancerous and may shorten the time for cancer to develop. Mutation Hotspots and Hereditary Cancer Risk In one koala, a copy of KoRV was found that had incorporated an entire cancer-related gene from the koala genome into its DNA sequence. This greatly increased expression of this gene and most likely caused cancer in this particular koala. If this mutated virus is transmissible, it would be of grave concern for koala conservation efforts. Comparing the genomic location of KoRVs between koalas also suggests that KoRV may predispose related koalas to particular tumors, with koalas sharing KoRV insertions in specific cancer-related genes suffering from similar types of cancer which they can pass on to their offspring. Across all koalas studied, there were “hot spots” in the genome where KoRV frequently inserts itself. These hot spots were also located in proximity to genes associated with cancer. “In summary then, we find multiple links at the genomic level between cancer-related genes and KoRV, revealing ways in which KoRV underlies the high frequency of cancer in koalas,” explains Gayle McEwen, scientist at the Leibniz-IZW. The results highlight the detrimental health consequences that wildlife species can suffer following germline infection by retroviruses. Germline invasions have been repeatedly experienced during vertebrate evolution and have shaped vertebrate genomes, including the lineage leading to modern humans. These were most likely associated with severe detrimental health effects, which must be endured and overcome to ensure species survival. The scientists at the Leibniz-IZW have previously shown that old retroviruses present in the koala genome aid the rapid degradation of KoRV. The koala finds itself in a race to survive the effects of KoRV long enough for the virus to be degraded. Considering the many threats to koalas, it is a race they need to win. Reference: “Retroviral integrations contribute to elevated host cancer rates during germline invasion” by Gayle K. McEwen, David E. Alquezar-Planas, Anisha Dayaram, Amber Gillett, Rachael Tarlinton, Nigel Mongan, Keith J. Chappell, Joerg Henning, Milton Tan, Peter Timms, Paul R. Young, Alfred L. Roca and Alex D. Greenwood, 26 February 2021, Nature Communications. DOI: 10.1038/s41467-021-21612-7 Scientists at the Princess Máxima Center and the Hubrecht Institute have developed brain organoids from human fetal brain tissue, offering new insights into brain development and disease modeling. These organoids, mirroring the complexity of the brain, could revolutionize research in neurology and oncology. (Artist’s concept.) Credit: SciTechDaily.com Scientists have developed 3D mini-organs from human fetal brain tissue that self-organize in vitro. These lab-grown organoids open up a brand-new way of studying how the brain develops. They also offer a valuable means to study the development and treatment of diseases related to brain development, including brain tumors. Researchers use different ways to model the biology of healthy tissue and disease in the lab. These include cell lines, laboratory animals and, since a few years, 3D mini-organs. These so-called organoids have characteristics and a level of complexity that allows scientists to closely model the functions of an organ in the lab. Organoids can be formed directly from cells of a tissue. Scientists can also ‘guide’ stem cells – found in embryos or in some adult tissues – to develop into the organ they aim to study. Advancements in Brain Organoid Development Until now, brain organoids were grown in the lab by coaxing embryonic or pluripotent stem cells to grow into structures representing different areas of the brain. Using a specific cocktail of molecules, they would try to mimic the natural development of the brain – with the ‘recipe’ for each cocktail taking a lot of research to develop. Now, scientists at the Princess Máxima Center for pediatric oncology and the Hubrecht Institute, both based in Utrecht, the Netherlands, developed brain organoids directly from human fetal brain tissue. The study was published in the prestigious journal Cell today (Monday), and was part-funded by the Dutch Research Council. An image of a whole human fetal brain organoid. Stem cells are marked by SOX2 (grey) and neuronal cells (TUJ1) are color coded from pink to yellow based on depth. Credit: Princess Máxima Center, Hubrecht Institute/B Artegiani, D Hendriks, H Clevers The researchers, led by Dr. Delilah Hendriks, Prof. Dr. Hans Clevers, and Dr. Benedetta Artegiani, were surprised to find that using small pieces of fetal brain tissue rather than individual cells was vital in growing mini-brains. To grow other mini-organs such as gut, scientists normally break down the original tissue to single cells. Instead working with small pieces of fetal brain tissue, the team found that these pieces could self-organize into organoids. The brain organoids were roughly the size of a grain of rice. The tissue’s 3D make-up was complex, and it contained a number of different types of brain cells. Importantly, the brain organoids contained many so-called outer radial glia – a cell type found in humans and our evolutionary ancestors. This underlines the organoids’ close similarity to – and use in studying – the human brain. The Significance of Extracellular Matrix The whole pieces of brain tissue also produced proteins that make up extracellular matrix – a kind of ‘scaffolding’ around cells. The team believes these proteins could be the reason why the pieces of brain tissue were able to self-organize into 3D brain structures. The presence of extracellular matrix in the organoids will allow further study of the environment of brain cells, and what happens when this goes wrong. A zoom-in image of a part of a human fetal brain organoid. Stem cells are marked by SOX2 (cyan) and neuronal cells (TUJ1) are color-coded from pink to yellow based on depth. Credit: Princess Máxima Center, Hubrecht Institute/B Artegiani, D Hendriks, H Clevers Potential for Brain Development and Cancer Research The researchers found that the tissue-derived organoids kept various characteristics of the specific region of the brain from which they were derived. They responded to signaling molecules known to play an important role in brain development. This finding suggests that the tissue-derived organoids could play an important role in untangling the complex network of molecules involved in directing the development of the brain. Given the ability of the tissue-derived organoids to quickly expand, the team next investigated their potential in modeling brain cancer. The researchers used gene-editing technique CRISPR-Cas9 to introduce faults in the well-known cancer gene TP53 in a small number of cells in the organoids. After three months, the cells with defective TP53 had completely overtaken the healthy cells in the organoid – meaning they had acquired a growth advantage, a typical feature of cancer cells. They then used CRISPR-Cas9 to switch off three genes linked to the brain tumor, glioblastoma: TP53, PTEN and NF1. The researchers also used these mutant organoids to look at their response to existing cancer drugs. These experiments showed the organoids’ potential for cancer drug research to link certain drugs to specific gene mutations. Four zoom-in images of parts of different human fetal brain organoids. Different neural markers are stained, depicting their cellular heterogeneity and architecture. Credit: Princess Máxima Center, Hubrecht Institute/B Artegiani, D Hendriks, H Clevers The tissue-derived organoids continued to grow in a dish for more than six months. Importantly, the scientists could multiply them, allowing them to grow many similar organoids from one tissue sample. The mini-tumors with the glioblastoma gene changes – were also capable of multiplying, keeping the same mix of mutations. This feature means scientists can carry out repeat experiments with the tissue-derived organoids, increasing the reliability of their findings. Next, the researchers aim to further explore the potential of their new tissue-derived brain organoids. They also plan to continue their work with bioethicists – who were already involved in shaping this research – to guide the future development and applications of the new brain organoids. Insights From Lead Researchers Dr. Benedetta Artegiani, research group leader at the Princess Máxima Center for pediatric oncology who co-led the research, says: “Brain organoids from fetal tissue are an invaluable new tool to study human brain development. We can now more easily study how the developing brain expands, and look at the role of different cell types and their environment. “Our new, tissue-derived brain model allows us to gain a better understanding of how the developing brain regulates the identity of cells. It could also help understand how mistakes in that process can lead to neurodevelopmental diseases such as microcephaly, as well as other diseases that can stem from derailed development, including childhood brain cancer.” Dr. Delilah Hendriks, affiliated group leader at the Princess Máxima Center for pediatric oncology, postdoctoral researcher at the Hubrecht Institute and Oncode Investigator, who co-led the research, says: “These new fetal tissue-derived organoids can offer novel insights into what shapes the different regions of the brain, and what creates cellular diversity. Our organoids are an important addition to the brain organoid field, that can complement the existing organoids made from pluripotent stem cells. We hope to learn from both models to decode the complexity of the human brain. “Being able to keep growing and using the brain organoids from fetal tissue also means that we can learn as much as possible from such precious material. We’re excited to explore the use of these novel tissue organoids for new discoveries about the human brain.” Prof. Dr. Hans Clevers, pioneer in organoid research and former research group leader at the Hubrecht Institute and the Princess Máxima Center for pediatric oncology and Oncode Investigator, co-led the research. He says: “With our study, we’re making an important contribution to the organoid and brain research fields. Since we developed the first human gut organoids in 2011, it’s been great to see that the technology has really taken off. Organoids have since been developed for almost all tissues in the human body, both healthy and diseased – including an increasing number of childhood tumors. “Until now, we were able to derive organoids from most human organs, but not from the brain – it’s really exciting that we’ve now been able to jump that hurdle as well.” Reference: “Human fetal brain self-organizes into long-term expanding organoids” by Delilah Hendriks, Anna Pagliaro, Francesco Andreatta, Ziliang Ma, Joey van Giessen, Simone Massalini, Carmen López-Iglesias, Gijs J.F. van Son, Jeff DeMartino, J. Mirjam A. Damen, Iris Zoutendijk, Nadzeya Staliarova, Annelien L. Bredenoord, Frank C.P. Holstege, Peter J. Peters, Thanasis Margaritis, Susana Chuva de Sousa Lopes, Wei Wu, Hans Clevers and Benedetta Artegiani, 8 January 2024, Cell. DOI: 10.1016/j.cell.2023.12.012 The study was performed in collaboration with Leiden University Medical Center, Utrecht University, Maastricht University, Erasmus University Rotterdam, and National University of Singapore. RRG455KLJIEVEWWF |
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