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KoDō 和牛燒肉值得排隊嗎?》公益路美食新手指南|10家必吃推薦 |
| 在地生活|大台北 2026/04/21 14:26:29 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人,臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」,從精緻西餐到創意火鍋,從日式丼飯到義式早午餐,每走幾步,就會有完全不同的特色料理餐廳。 這次我特別花了一整個月,實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店,也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準,整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你,找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型,從高質感牛排館到巷弄系早午餐,每一間都有自己獨特的風格。為了讓整體比較更客觀,我依照以下四大面向進行評比,並搭配實際用餐體驗來打分。
整體而言,我希望這份評比不只是「哪家好吃」,而是幫你在不同情境下(約會、家庭聚餐、朋友小聚、商業午餐)都能快速找到合適的選擇。畢竟,美食不只是味覺的滿足,更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段,從火鍋、燒肉到中式料理與早午餐,每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單,橫跨平價、創意、高級各路風格。
一頭牛日式燒肉|炭香濃郁的和牛饗宴,約會聚餐首選
走在公益路上,很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面,搭配昏黃燈光與暖色調的內裝,讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大,但桌距規劃得宜,每桌皆設有獨立排煙設備,烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂,絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網,讓人完全不用分心。整場用餐過程就像一場表演,從視覺、嗅覺到味覺都被滿足。 綜合評分
地址:408臺中市南屯區公益路二段162號電話:04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」,更像是一場五感盛宴。從進門那一刻到最後一道甜點,都能感受到他們對細節的用心。 TANG Zhan 湯棧|文青系火鍋代表,麻香湯底與視覺美感並重
在公益路這條美食戰線上,TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址:408臺中市南屯區公益路二段248號電話:04-22580617 官網:https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度,又結合現代風格與細節服務,讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店,湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店|明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂,那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主,陽光灑進室內,讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧,建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味,選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口,米芯保留微Q口感;而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香,口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹,無論是一個人帶電腦工作、或朋友聚餐,都能找到舒服角落。餐點上桌速度穩定,服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」,很適合想遠離忙碌日常的人。 綜合評分
地址:40861臺中市南屯區公益路二段18號電話:04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇,而是以「剛剛好」的份量與風味,陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店,NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物|平價卻用心的湯頭系火鍋,家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上,加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐,但靠著實在的湯頭與親切的服務,默默吸引許多回頭客。每到用餐時間,總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底,湯頭清澈卻不單薄,越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向,桌距寬敞、座位舒適,帶小孩來也不覺擁擠。店員態度親切,補湯、收盤都很勤快,給人一種「被照顧著」的安心感。 綜合評分
地址:403臺中市西區公益路288號電話:0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤,而是用最簡單的湯頭與新鮮食材,傳遞出家常卻不平凡的溫度。 印月餐廳|中式料理的藝術演繹,宴客與家庭聚會首選
說到臺中公益路的中式料理代表,印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名,把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設,每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏,都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法,讓人感受到「被款待」的尊榮感。 綜合評分
地址:408臺中市南屯區公益路二段818號電話:0422511155 小結語印月餐廳是一間「不只吃飯,更像品味生活」的地方。 KoDō 和牛燒肉|極致職人精神,專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗,一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉,以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址:403臺中市西區公益路260號電話:0423220312 官網:https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳,而是一場體驗。 永心鳳茶|在茶香裡用餐的優雅時光,臺味早午餐的新詮釋
走進 永心鳳茶公益店,彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤,無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」,都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和,對茶品介紹詳盡。上餐節奏剛好,不急不徐。 綜合評分
地址:40360臺中市西區公益路68號三樓(勤美誠品)電話:0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓|老饕級江浙功夫菜,穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主,兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址:408臺中市南屯區公益路二段95號電話:0423202322 官網:https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司|低調奢華的無菜單日料,職人手藝詮釋旬味極致
在熱鬧的公益路上,一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase(無菜單料理) 形式,每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘,節奏緩慢但沉穩。 綜合評分
地址:408臺中市南屯區公益路二段25號電話:0423206368 官網:https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂|人氣爆棚的和牛燒肉聖地,肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳,茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐,價格約落在 $700–$1000 間,份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快,整場用餐流程流暢無壓力。 綜合評分
地址:403臺中市西區公益路268號電話:0423281167 官網:https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質+輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後,臺中公益路不只是一條美食街,而是一段生活風景線。 有的餐廳講究細膩與儀式感,像 一頭牛日式燒肉 與 一笈壽司,讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心,像 加分昆布鍋物、永心鳳茶,讓人明白吃飯不只是為了飽足,而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店,則用穩定的品質與熱絡的氛圍,成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店,構成了公益路最動人的縮影 有華麗的,也有溫柔的;有傳統的,也有創新的。 每一家都在自己的風格裡發光,讓人吃到的不只是料理,而是一種生活的溫度與節奏。 對我而言,這不僅是一場美食旅程,更是一趟關於「臺中味道」的回憶之旅。 FAQ:關於臺中公益路美食常見問題Q1:公益路哪一區的餐廳最集中? Q2:需要提前訂位嗎? 最後的話若要用一句話形容這趟美食之旅,我會說: NINI 尼尼臺中店長輩會喜歡嗎? 如果你也和我一樣喜歡用味蕾探索一座城市,那就把這篇公益路美食攻略收藏起來吧。印月餐廳套餐劃算嗎? 無論是約會、慶生、家庭聚餐,或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。加分100%浜中特選昆布鍋物甜點好吃嗎? 下一餐,不妨從這10家開始。印月餐廳適合辦尾牙嗎? 打開手機、約上朋友,讓公益路成為你生活裡最容易抵達的小確幸。加分100%浜中特選昆布鍋物用餐時間會不會太短? 如果你有私心愛店,也歡迎留言分享,TANG Zhan 湯棧座位舒適嗎? 你的推薦,可能讓我下一趟美食旅程變得更精彩。TANG Zhan 湯棧口味偏臺式還是日式? Yale scientists have reprogrammed the genetic code of an organism, creating a novel genomically recoded organism (GRO) with only one stop codon, enabling the production of synthetic proteins with new functions. This breakthrough paves the way for advanced biotherapeutics and biomaterials with vast medical and industrial applications. Yale researchers have created “Ochre,” a genomically recoded organism that enables the production of synthetic proteins with novel properties, paving the way for groundbreaking applications in medicine, biotechnology, and industry. Synthetic biologists from Yale successfully rewrote the genetic code of an organism—a novel genomically recoded organism (GRO) with a single stop codon—using a cellular platform they developed that enables the production of new classes of synthetic proteins. Researchers say these synthetic proteins offer the promise of innumerable medical and industrial applications that can benefit society and human health. A new study published in the journal Nature describes the creation of the landmark GRO, known as “Ochre,” which fully compresses redundant (or “degenerate”) codons into a single codon. A codon is a sequence of three nucleotides in DNA or RNA that codes for a specific amino acid, which serves as the biochemical building block for proteins. “This research allows us to ask fundamental questions about the malleability of genetic codes,” said Farren Isaacs, professor of molecular, cellular, and developmental biology at Yale School of Medicine and of biomedical engineering at Yale’s Faculty of Arts and Sciences, who is co-senior author of the paper. “It also demonstrates the ability to engineer the genetic code to endow multi-functionality into proteins and usher in a new era of programmable biotherapeutics and biomaterials.” Building on Past Breakthroughs in Genomic Recoding The landmark advance builds on a 2013 study by the team, published in Science, which described the construction of the first GRO. In that study, the researchers demonstrated new solutions for safeguarding genetically engineered organisms and for producing new classes of synthetic proteins and biomaterials with “unnatural,” or human-created, chemistries. Ochre is a major step toward creating a non-redundant genetic code in E. coli, specifically, which is ideally suited to produce synthetic proteins containing multiple, different synthetic amino acids. A codon, a sequence of three nucleotides in DNA and RNA that codes for a specific amino acid, acts like an “instruction manual” for protein synthesis, telling the cell which of the 20 natural amino acids to add to a growing protein chain — or, in the case of the three “stop” codons (known as TAG, TGA, and TAA), signaling the termination of protein synthesis. Yale scientists recoded a cell to have a single, non-degenerative TAA codon. The newly “free” TGA and TAG codons have been reassigned to encode nonstandard amino acids into synthetic proteins that possess new chemistries with innumerable applications. Credit: Yale University / Michael S. Helfenbein Jesse Rinehart, an associate professor of cellular and molecular physiology at the Yale School of Medicine and co-senior author on the study, called the breakthrough a “profound piece of whole genome engineering based on over 1,000 precise edits at a scale an order of magnitude greater than any engineering feat we have previously done.” “This is an exciting new platform technology that opens up an array of applications for biotechnology both in the academic realm and in the commercial sector,” Rinehart said. “We want to advance our general knowledge of science but we also want to enable industrial applications that are beneficial to society.” The codon, a sequence of three nucleotides in DNA or RNA, acts like an “instruction manual” for protein synthesis, telling the cell which of the 20 natural amino acids to add to a growing protein chain (or, in the case of “stop” codons, signaling the termination of protein synthesis). In this process, known as translation, the genetic information carried in a messenger RNA (mRNA), via the genetic code, dictates not only the order of amino acids but also when the process should start and stop. Reprogramming the Genetic Code for Novel Functions Michael Grome, a postdoctoral associate in molecular, cellular, and developmental biology at Yale and first author of the study, likened codons to three-letter words within a sentence in the genetic recipe for life. Inside the cell, he said, there are ribosomes that act like 3-D printers that read the recipe. Each word calls for one “ingredient” amino acid from among the list of 20 natural amino acids that make up proteins. “A lot of these words are equivalent, or synonymous,” Grome said. “We set out to add more ingredients for building proteins, so we took three of these words for ‘stop’ and made them one. Two words were removed, then we re-engineered the cell so they were ‘freed’ for new function. We then engineered a cell that recognized the word to say something new, to represent a new ingredient.” Specifically, the researchers eliminated two of the three stop codons that terminate protein production. The recoded genome reassigned four codons to non-degenerate functions, including the two recoded stop codons dedicated to encoding nonstandard, or unnatural, amino acids into protein. In addition to introducing thousands of precise edits across the genome, the work required AI-guided design and re-engineering of essential protein and RNA translation factors to create a strain capable of adding two nonstandard amino acids into its recipe book. These nonstandard amino acids imbue proteins with multiple new properties, such as programmable biologics with reduced immunogenicity (a substance’s ability to induce an immune response in the body) or biomaterials with enhanced conductivity. The results reflect years of recoding work by the two labs at the Yale Systems Biology Institute on West Campus. The collaboration between Rinehart and Isaacs dates to 2010 when they began working in neighboring labs. Isaacs has long been interested in engineering genomes — much like, he said, an architect might plan and make changes to a building. Rinehart’s work focuses on proteins — how they are made and how the stage might be set for them to carry out other actions. “We recognized we have complementary expertise and that both labs bring a broad set of expertise and capability,” Rinehart said. Isaacs is excited about what he describes as the potentially “killer” applications for programmable protein biologics that the new platform will make possible. One such application involves engineering protein drugs with synthetic chemistries to decrease the frequency of dosing or undesirable immune responses. The team reported such an application using their first-generation GRO in a 2022 study. In that study they encoded non-standard amino acids into protein, demonstrating a safer, controllable approach to precisely tune the half-life of protein biologics. The new Ochre cell expands these capabilities for use in the construction of multi-functional biologics. Isaacs and Rinehart are currently acting as advisors to Pear Bio, a Yale biotechnology spin-off that has licensed the technology for commercializing programmable biologics. Reference: “Engineering a genomically recoded organism with one stop codon” by Michael W. Grome, Michael T. A. Nguyen, Daniel W. Moonan, Kyle Mohler, Kebron Gurara, Shenqi Wang, Colin Hemez, Benjamin J. Stenton, Yunteng Cao, Felix Radford, Maya Kornaj, Jaymin Patel, Maisha Prome, Svetlana Rogulina, David Sozanski, Jesse Tordoff, Jesse Rinehart and Farren J. Isaacs, 5 February 2025, Nature. DOI: 10.1038/s41586-024-08501-x Image showing the human small intestine. Credit: Grace Burgin, Noga Rogel & Moshe Biton, Klarman Cell Observatory, Broad Institute New research from the Human Cell Atlas offers insights into cell development, disease mechanisms, and genetic influences, enhancing our understanding of human biology and health. The Human Cell Atlas (HCA) consortium has made significant progress in its mission to better understand the cells of the human body in health and disease, with a recent publication of a Collection of more than 40 peer-reviewed papers in Nature and other Nature Portfolio journals. The Collection showcases a range of large-scale datasets, artificial intelligence algorithms, and biomedical discoveries from the HCA that are enhancing our understanding of the human body. The studies reveal insights into how the placenta and skeleton form, changes during brain maturation, new gut and vascular cell states, lung responses to COVID-19, and the effects of genetic variation on disease, among others. Contributed by researchers worldwide, the papers in the Collection serve as essential tools and examples for building cell atlases on a large scale. Collectively, they demonstrate the HCA’s commitment to capturing the full spectrum of human diversity, including genetic, geographic, age, and sex differences. Skin organoid showing hair follicles with endothelial cells. Credit: Haniffa et al. DOI 10.1038s41586-024-08002-x Comprehensive Mapping of Human Cells The HCA is developing and using experimental and computational approaches in single-cell and spatial genomics to create comprehensive reference maps of all human cells—the fundamental units of life—as a basis for both understanding human health and diagnosing, monitoring, and treating disease. To date, more than 3,600 HCA members from over 100 countries have worked together to profile more than 100 million cells from over 10,000 people. Researchers are currently working to assemble a first draft Human Cell Atlas, which will eventually grow to include up to billions of cells across all organs and tissues. Human lung tissue. Credit: Nathan Richoz University of Cambridge New Insights from the HCA Collection This Collection of studies in Nature Portfolio demonstrates major advances in three aspects of HCA’s mission: mapping individual adult tissues or organs, mapping developing human tissues, and developing groundbreaking new analytical methods, including artificial intelligence/machine learning-based methods. The researchers involved are members of the 18 Biological Networks of the HCA, each of which is focused on a particular organ, tissue, or system. The 18 biological networks that the Human Cell Atlas work is investigating. Credit: Ania Hupalowska Foundational Goals and Achievements of HCA “The Human Cell Atlas is a global initiative that is already transforming our understanding of human health. By creating a comprehensive reference map of the healthy human body—a kind of ‘Google Maps’ for cell biology—it establishes a benchmark for detecting and understanding the changes that underlie health and disease. This new level of insight into the specific genes, mechanisms, and cell types within tissues is laying the groundwork for more precise diagnostics, innovative drug discovery and advanced regenerative medicine approaches,” said Professor Sarah Teichmann, founding co-chair of the Human Cell Atlas, now at the Cambridge Stem Cell Institute. Professor Sarah Teichmann, founding co-Chair of the Human Cell Atlas, now at the Cambridge Stem Cell Institute. Credit: Wellcome Sanger Institute Enhancing Our Understanding of Human Biology Dr. Aviv Regev, founding co-chair of the HCA, now at Genentech, said: “This is a pivotal moment for the HCA community as we move towards achieving the first draft of the Human Cell Atlas. This collection of studies showcases the major advances from biology to AI achieved since the publication of the HCA White Paper in 2017 and that now deliver numerous biological and clinical insights. This large-scale, community-driven, globally representative, and rigorously curated atlas will evolve continuously and remain accessible to all to advance our understanding of the human body in health and treatments for disease.” Dr. Aviv Regev, founding co-Chair of the HCA. Credit: Genentech Detailed Insights into Human Tissues and Disease Several studies in the Collection provide a detailed analysis of specific tissues and organs and reveal new biological discoveries important for understanding disease. For example, a cell atlas of the human gut from healthy and diseased tissue identified a gut cell type that may be involved in gut inflammation [Oliver et al.], providing a valuable resource for investigating and ultimately treating conditions such as ulcerative colitis and Crohn’s disease. Developmental Biology and Genetic Insights The new collection of papers also includes novel maps of human tissues during development. These include the first map of human skeletal development, revealing how the skeleton forms [To et al.], shedding light on the origins of arthritis, and identifying cells involved in skeletal conditions. An additional study describes a multi-omic atlas of the first-trimester placenta, including insight into genetic programs that control how the placenta develops and functions to provide nutrients and protection to the embryo [Shu et al.]. These and other developmental biology studies in the Collection increase our fundamental understanding of healthy development in time and space and provide blueprints and resources for creating therapeutics since many diseases originate in human development. Promoting Equity and Ethical Research An accompanying article highlights the importance of including samples from historically underrepresented human populations and describes actions and principles aimed at promoting equitable science [Amit et al.]. Professor Partha Majumder of the John C Martin Centre for Liver Research and Innovation, India, and a member of the HCA Organizing Committee member and Co-Chair of the HCA Equity Working Group, said: “A key priority for HCA is to ensure a representation of the vast range of human diversity; genetic, cultural and geographical. HCA studies such as the Asian Immune Diversity Atlas and the analysis of distinctive histopathological differences in COVID-19 samples from Malawi demonstrate the remarkable power of large-scale international scientific collaboration.” Another article illustrates HCA’s role in developing new ethical guidance on a broad range of issues in genomic science and making this advice available to scientists worldwide [Kirby et al.]. AI Revolutionizing Cellular Biology Research Just as AI has revolutionized humans’ ability to process text quickly, it is also now helping scientists to develop a deeper and more complete understanding of biology at the cellular level and beyond. The Collection introduces new AI methods to better understand and classify cell types and search for cells in this vast map. For example, SCimilarity [Heimberg et al.] enables researchers to compare single-cell datasets to identify similar cell types in different tissues and contexts, analogous to how “reverse image search” can search for photos. Other research teams tackled long-standing challenges, such as classifying cells into hierarchical groups based on their properties, known as cell annotation [e.g., Ergan et al. and Fischer et al.] Conclusion: Impact of the HCA Collection Dr. Jeremy Farrar, Chief Scientist, World Health Organization, said: “This landmark collection of papers from the international Human Cell Atlas community underscores the tremendous progress toward mapping every single kind of human cell and how they change as we grow up and age. The insights emerging from these discoveries are already reshaping our understanding of health and disease, paving the way for transformative health benefits that will impact lives worldwide.” Reference: “The Human Cell Atlas: towards a first draft atlas” 20 November 2024, Nature. The individual studies in the Collection were funded by over one hundred different funding sources worldwide. The HCA also receives organizational support from the Chan Zuckerberg Initiative, Wellcome, the Klarman Family Foundation, the Helmsley Charitable Trust, and others. Recent research reveals a single mutation in a critical protein structure, the synaptonemal complex, can cause male infertility. This discovery, made through gene editing in mice, opens new possibilities for understanding and treating male infertility. Scientists at Stowers Institute collaborate to discover an underlying cause of male infertility. Infertility affects countless couples globally, and in half of these cases, the problem lies with the male partner. Specifically, about 10% of these men face the challenge of producing minimal or no sperm. Recent research conducted jointly by the Stowers Institute for Medical Research and the Wellcome Centre for Cell Biology at the University of Edinburgh are providing insights into the malfunctions occurring during sperm development. This research opens doors to new hypotheses regarding potential treatment methods. “A significant cause of infertility in males is that they just cannot make sperm,” said Stowers Investigator Scott Hawley, Ph.D. “If you know exactly what is wrong, there are technologies emerging right now that might give you a way to fix it.” The study recently published in Science Advances from the Hawley Lab and Wellcome Centre Investigator Owen Davies, Ph.D., may help explain why some men do not make enough sperm to fertilize an egg. In most sexually reproducing species, including humans, a critical protein structure resembling a lattice-like bridge needs to be built properly to produce sperm and egg cells. The team led by former Postdoctoral Research Associate Katherine Billmyre, Ph.D., discovered that in mice, changing a single and very specific point in this bridge caused it to collapse, leading to infertility and thus providing insight into human infertility in males due to similar problems with meiosis. A video explaining the findings. Credit: Stowers Institute for Medical Research The Role of Meiosis in Reproductive Health Meiosis, the cell division process giving rise to sperm and eggs, involves several steps, one of which is the formation of a large protein structure called the synaptonemal complex. Like a bridge, the complex holds chromosome pairs in place enabling necessary genetic exchanges to occur that are essential for the chromosomes to then correctly separate into sperm and eggs. “A significant contributor to infertility is defects in meiosis,” said Billmyre. “To understand how chromosomes separate into reproductive cells correctly, we are really interested in what happens right before that when the synaptonemal complex forms between them.” Microscopy images showing normal seminiferous tubules in control testes with mature sperm (black arrow: left) but smaller empty seminiferous tubules in testes harboring a synaptonemal complex protein point mutation (black asterisk: right). Credit: Stowers Institute for Medical Research Previous studies have examined many proteins comprising the synaptonemal complex, how they interact with each other, and have identified various mutations linked to male infertility. The protein the researchers investigated in this study forms the lattices of the proverbial bridge, which has a section found in humans, mice, and most other vertebrates suggesting it is critical for assembly. Modeling different mutations in a potentially crucial region in the human protein enabled the team to predict which of these might disrupt protein function. The authors used a precise gene editing technique to make mutations in one key synaptonemal complex protein in mice, which allowed the researchers, for the first time, to test the function of key regions of the protein in live animals. Just a single mutation, predicted from the modeling experiments, was verified as the culprit of infertility in mice. Representative testes from 9-week-old control mice (left) and mice with a point mutation in one synaptonemal complex protein (right). Credit: Stowers Institute for Medical Research “We’re talking about pinpoint surgery here,” said Hawley. “We focused on a tiny little region of one protein in this gigantic structure that we were pretty sure could be a significant cause of infertility.” Implications for Human Health Mice have long been used as models for human diseases. From the modeling experiments using human protein sequences, along with the high conservation of this protein structure across species, the precise molecule that caused infertility in mice likely functions the same way in humans. “What is really exciting to me is that our research can help us understand this really basic process that is necessary for life,” said Billmyre. Model of the synaptonemal complex in control and mutant mice. The protein the team investigated (SYCP1) forms normally, and all additional necessary proteins are recruited. In the mutant, SYCP1 localizes to the chromosome axes but does not successfully form the bridge-like structure (head-to-head interactions), and the additional proteins that help keep the bridge intact are either missing or not properly organized. Credit: Stowers Institute for Medical Research For Hawley, this research is a true representation of the versatility of the Institute. Hawley’s lab typically conducts research in fruit flies, yet the protein discovered in this study was not present in fruit flies and demanded a different research organism to continue. Because of the resources and Technology Centers at the Institute, it was possible to quickly pivot and test the new infertility hypothesis in mice. “I can’t imagine another place where this could happen,” said Hawley. “I think it’s an amazing example of how the Stowers Institute’s dedication toward discovery can yield big results providing important leaps forward in understanding.” Reference: “SYCP1 head-to-head assembly is required for chromosome synapsis in mouse meiosis” by Katherine Kretovich Billmyre, Emily A. Kesler, Dai Tsuchiya, Timothy J. Corbin, Kyle Weaver, Andrea Moran, Zulin Yu, Lane Adams, Kym Delventhal, Michael Durnin, Owen Richard Davies and R. Scott Hawley, 20 October 2023, Science Advances. DOI: 10.1126/sciadv.adi1562 Additional authors include Emily A. Kesler, Dai Tsuchiya, Ph.D., Timothy J. Corbin, Kyle Weaver, Andrea Moran, Zulin Yu, Ph.D., Lane Adams, Kym Delventhal, Michael Durnin, Ph.D., and Owen Richard Davies, Ph.D. This work was funded by the Wellcome Centre for Cell Biology (award: 203149), the Wellcome Senior Research Fellowship (award: 219413/Z/19/Z), and by institutional support from the Stowers Institute for Medical Research. RRG455KLJIEVEWWF |
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