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文章數：84 |
 茶六燒肉堂慶生氛圍夠嗎？》台中公益路聚餐推薦｜10大類型餐廳評比 |
| 心情隨筆｜心情日記 2026/04/19 02:25:06 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人，臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」，從精緻西餐到創意火鍋，從日式丼飯到義式早午餐，每走幾步，就會有完全不同的特色料理餐廳。 這次我特別花了一整個月，實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店，也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準，整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你，找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型，從高質感牛排館到巷弄系早午餐，每一間都有自己獨特的風格。為了讓整體比較更客觀，我依照以下四大面向進行評比，並搭配實際用餐體驗來打分。
整體而言，我希望這份評比不只是「哪家好吃」，而是幫你在不同情境下（約會、家庭聚餐、朋友小聚、商業午餐）都能快速找到合適的選擇。畢竟，美食不只是味覺的滿足，更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段，從火鍋、燒肉到中式料理與早午餐，每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單，橫跨平價、創意、高級各路風格。
一頭牛日式燒肉｜炭香濃郁的和牛饗宴，約會聚餐首選
走在公益路上，很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面，搭配昏黃燈光與暖色調的內裝，讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大，但桌距規劃得宜，每桌皆設有獨立排煙設備，烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂，絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網，讓人完全不用分心。整場用餐過程就像一場表演，從視覺、嗅覺到味覺都被滿足。 綜合評分
地址：408臺中市南屯區公益路二段162號電話：04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」，更像是一場五感盛宴。從進門那一刻到最後一道甜點，都能感受到他們對細節的用心。 TANG Zhan 湯棧｜文青系火鍋代表，麻香湯底與視覺美感並重
在公益路這條美食戰線上，TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址：408臺中市南屯區公益路二段248號電話：04-22580617 官網：https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度，又結合現代風格與細節服務，讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店，湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店｜明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂，那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主，陽光灑進室內，讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧，建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味，選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口，米芯保留微Q口感；而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香，口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹，無論是一個人帶電腦工作、或朋友聚餐，都能找到舒服角落。餐點上桌速度穩定，服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」，很適合想遠離忙碌日常的人。 綜合評分
地址：40861臺中市南屯區公益路二段18號電話：04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇，而是以「剛剛好」的份量與風味，陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店，NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物｜平價卻用心的湯頭系火鍋，家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上，加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐，但靠著實在的湯頭與親切的服務，默默吸引許多回頭客。每到用餐時間，總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底，湯頭清澈卻不單薄，越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向，桌距寬敞、座位舒適，帶小孩來也不覺擁擠。店員態度親切，補湯、收盤都很勤快，給人一種「被照顧著」的安心感。 綜合評分
地址：403臺中市西區公益路288號電話：0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤，而是用最簡單的湯頭與新鮮食材，傳遞出家常卻不平凡的溫度。 印月餐廳｜中式料理的藝術演繹，宴客與家庭聚會首選
說到臺中公益路的中式料理代表，印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名，把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設，每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏，都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法，讓人感受到「被款待」的尊榮感。 綜合評分
地址：408臺中市南屯區公益路二段818號電話：0422511155 小結語印月餐廳是一間「不只吃飯，更像品味生活」的地方。 KoDō 和牛燒肉｜極致職人精神，專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗，一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉，以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址：403臺中市西區公益路260號電話：0423220312 官網：https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳，而是一場體驗。 永心鳳茶｜在茶香裡用餐的優雅時光，臺味早午餐的新詮釋
走進 永心鳳茶公益店，彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤，無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」，都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和，對茶品介紹詳盡。上餐節奏剛好，不急不徐。 綜合評分
地址：40360臺中市西區公益路68號三樓(勤美誠品)電話：0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓｜老饕級江浙功夫菜，穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主，兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址：408臺中市南屯區公益路二段95號電話：0423202322 官網：https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司｜低調奢華的無菜單日料，職人手藝詮釋旬味極致
在熱鬧的公益路上，一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase（無菜單料理） 形式，每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘，節奏緩慢但沉穩。 綜合評分
地址：408臺中市南屯區公益路二段25號電話：0423206368 官網：https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂｜人氣爆棚的和牛燒肉聖地，肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳，茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐，價格約落在 $700–$1000 間，份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快，整場用餐流程流暢無壓力。 綜合評分
地址：403臺中市西區公益路268號電話：0423281167 官網：https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質＋輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後，臺中公益路不只是一條美食街，而是一段生活風景線。 有的餐廳講究細膩與儀式感，像 一頭牛日式燒肉 與 一笈壽司，讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心，像 加分昆布鍋物、永心鳳茶，讓人明白吃飯不只是為了飽足，而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店，則用穩定的品質與熱絡的氛圍，成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店，構成了公益路最動人的縮影 有華麗的，也有溫柔的；有傳統的，也有創新的。 每一家都在自己的風格裡發光，讓人吃到的不只是料理，而是一種生活的溫度與節奏。 對我而言，這不僅是一場美食旅程，更是一趟關於「臺中味道」的回憶之旅。 FAQ：關於臺中公益路美食常見問題Q1：公益路哪一區的餐廳最集中？ Q2：需要提前訂位嗎？ 最後的話若要用一句話形容這趟美食之旅，我會說： 加分100%浜中特選昆布鍋物家庭過節聚會適合嗎？ 如果你也和我一樣喜歡用味蕾探索一座城市，那就把這篇公益路美食攻略收藏起來吧。一頭牛日式燒肉尾牙預算好掌控嗎？ 無論是約會、慶生、家庭聚餐，或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。NINI 尼尼臺中店需要訂位嗎？ 下一餐，不妨從這10家開始。一頭牛日式燒肉單點比較好嗎？ 打開手機、約上朋友，讓公益路成為你生活裡最容易抵達的小確幸。KoDō 和牛燒肉套餐劃算嗎？ 如果你有私心愛店，也歡迎留言分享，永心鳳茶慶生氣氛夠嗎？ 你的推薦，可能讓我下一趟美食旅程變得更精彩。NINI 尼尼臺中店情侶來合適嗎？ This is a visual representation of the simulated Pong environment where neuron activity is reflected in the tiles growing in height. Credit: Kagan et al. / Neuron Live biological neurons show more about how a brain works than AI ever will. Scientists have shown for the first time that 800,000 brain cells living in a dish can perform goal-directed tasks. In this case, they played the simple tennis-like computer game, Pong. The results of the Melbourne-led study are published today (October 12) in the journal Neuron. Now the researchers are going to investigate what happens when their DishBrain is affected by medicines and alcohol. “We have shown we can interact with living biological neurons in such a way that compels them to modify their activity, leading to something that resembles intelligence,” says lead author Dr. Brett Kagan. He is Chief Scientific Officer of biotech start-up Cortical Labs, which is dedicated to building a new generation of biological computer chips. His co-authors are affiliated with Monash University, RMIT University, University College London, and the Canadian Institute for Advanced Research. A microscopy image of neural cells where fluorescent markers show different types of cells. Green marks neurons and axons, purple marks neurons, red marks dendrites, and blue marks all cells. Where multiple markers are present, colors are merged and typically appear as yellow or pink depending on the proportion of markers, credit Cortical Labs. Credit: Cortical Labs “DishBrain offers a simpler approach to test how the brain works and gain insights into debilitating conditions such as epilepsy and dementia,” says Dr. Hon Weng Chong, Chief Executive Officer of Cortical Labs. Understanding Brain Function Through DishBrain Although researchers have been able to mount neurons on multi-electrode arrays and read their activity for some time now, this is the first time that cells have been stimulated in a structured and meaningful way. “In the past, models of the brain have been developed according to how computer scientists think the brain might work,” Kagan says. “That is usually based on our current understanding of information technology, such as silicon computing. “But in truth, we don’t really understand how the brain works.” This video shows the game Pong being controlled by a layer of neurons in a dish. Credit: Kagan et. al / Neuron By constructing a living model brain from basic structures in this way, scientists will be able to experiment using real brain function rather than flawed analogous models such as a computer. For example, Kagan and his team will next experiment to see what effect alcohol has when introduced to DishBrain. “We’re trying to create a dose-response curve with ethanol – basically get them ‘drunk’ and see if they play the game more poorly, just as when people drink,” says Kagan. That may pave the way for completely new methods of understanding what is happening with the brain. Scanning Electron Microscope image of a neural culture that has been growing for more than six months on a high-density multi-electrode array. A few neural cells grow around the periphery and have developed complicated networks which cover the electrodes in the center. Credit Cortical Labs Potential for Revolutionizing Brain Research and Technology “This new capacity to teach cell cultures to perform a task in which they exhibit sentience – by controlling the paddle to return the ball via sensing – opens up new discovery possibilities which will have far-reaching consequences for technology, health, and society,” says Dr. Adeel Razi. He is the Director of Monash University’s Computational & Systems Neuroscience Laboratory. “We know our brains have the evolutionary advantage of being tuned over hundreds of millions of years for survival. Now, it seems we have in our grasp where we can harness this incredibly powerful and cheap biological intelligence.” Cortical Labs Chief Scientific Officer, Dr. Brett J. Kagan (seated), and Chief Executive Officer, Dr. Hon Weng (standing), conducting cell work on multielectrode arrays in a biosafety hood. Credit: Cortical Labs The findings also raise the possibility of creating an alternative to animal testing when investigating how new drugs or gene therapies respond in these dynamic environments. “We have also shown we can modify the stimulation based on how the cells change their behavior and do that in a closed-loop in real-time,” says Kagan. Brett Kagan, Chief Scientific Officer, Cortical Labs. Credit: Cortical Labs To perform the experiment, the team of scientists gathered mouse cells from embryonic brains as well as some human brain cells derived from stem cells. They grew them on top of microelectrode arrays that could both stimulate them and read their activity. Electrodes on the left or right of one array were fired to tell Dishbrain which side the ball was on, while the distance from the paddle was indicated by the frequency of signals. Feedback from the electrodes taught DishBrain how to return the ball, by making the cells act as if they themselves were the paddle. The Beauty of Interactive Neuron Systems “We’ve never before been able to see how the cells act in a virtual environment,” says Kagan. “We managed to build a closed-loop environment that can read what’s happening in the cells, stimulate them with meaningful information, and then change the cells in an interactive way so they can actually alter each other.” “The beautiful and pioneering aspect of this work rests on equipping the neurons with sensations — the feedback — and crucially the ability to act on their world,” says co-author Professor Karl Friston, a theoretical neuroscientist at UCL, London. “Remarkably, the cultures learned how to make their world more predictable by acting upon it. This is remarkable because you cannot teach this kind of self-organization; simply because — unlike a pet — these mini-brains have no sense of reward and punishment,” he says. Translational Potential: Testing Drugs in Real-Time “The translational potential of this work is truly exciting: it means we don’t have to worry about creating ‘digital twins’ to test therapeutic interventions. We now have, in principle, the ultimate biomimetic ‘sandbox’ in which to test the effects of drugs and genetic variants – a sandbox constituted by exactly the same computing (neuronal) elements found in your brain and mine.” The research also supports the “free energy principle” developed by Professor Friston. “We faced a challenge when we were working out how to instruct the cells to go down a certain path. We don’t have direct access to dopamine systems or anything else we could use to provide specific real-time incentives so we had to go a level deeper to what Professor Friston works with: information entropy – a fundamental level of information about how the system might self-organize to interact with its environment at the physical level. “The free energy principle proposes that cells at this level try to minimize the unpredictability in their environment.” Kagan says one exciting finding was that DishBrain did not behave like silicon-based systems. “When we presented structured information to disembodied neurons, we saw they changed their activity in a way that is very consistent with them actually behaving as a dynamic system,” he says. “For example, the neurons’ ability to change and adapt their activity as a result of experience increases over time, consistent with what we see with the cells’ learning rate.” Chong says he was excited by the discovery, but it was just the beginning. “This is brand new, virgin territory. And we want more people to come on board and collaborate with this, to use the system that we’ve built to further explore this new area of science,” he says. “As one of our collaborators said, it’s not every day that you wake up and you can create a new field of science.” Reference: “In vitro neurons learn and exhibit sentience when embodied in a simulated game-world” by Brett J. Kagan, Andy C. Kitchen, Nhi T. Tran, Forough Habibollahi, Moein Khajehnejad, Bradyn J. Parker, Anjali Bhat, Ben Rollo, Adeel Razi and Karl J. Friston, 12 October 2022, Neuron. DOI: 10.1016/j.neuron.2022.09.001 B.J.K. is an employee of Cortical Labs. B.J.K. and A.C.K. are shareholders of Cortical Labs. B.J.K. and A.C.K. hold an interest in patents related to this publication. F.H. and M.K. received funding from Cortical Labs for work related to this publication. Sperm illustration. University of Toledo research shows the centriole’s role in sperm evolved from a shock absorber to a transmission system. Scientists at The University of Toledo discovered a new movement in sperm that provides innovative avenues for diagnostics and therapeutic strategies for male infertility. The research published in Nature Communications finds that the atypical centriole in the sperm neck acts as a transmission system that controls twitching in the head of the sperm, mechanically synchronizing the sperm tail movement to the new head movement. The centriole has historically been considered a rigid structure that acts like a shock absorber. “We think the atypical centriole in the sperm’s neck is an evolutionary innovation whose function is to make your sperm move better,” said Dr. Tomer Avidor-Reiss, professor of biological sciences in the UToledo College of Natural Sciences and Mathematics. “Reproductive success depends on the ability of sperm to swim through female reproductive tract barriers while out-competing their rivals to fertilize the egg.” Ph.D. candidate Sushil Khanal, left, and Dr. Tomer Avidor-Reiss, professor of biological sciences, pull cryopreserved semen samples out of a liquid nitrogen tank that is kept at -196 degrees Celsius. The researchers found that the role of the centriole in sperm evolved from acting as a shock absorber to a transmission system, a discovery that could lead to innovative ways to help diagnose and treat male infertility. Credit: Daniel Miller, The University of Toledo The study led by Ph.D. candidate Sushil Khanal builds upon the lab’s previous groundbreaking discovery in human sperm that changed the dogma in reproductive biology: A father donates not one but two centrioles through the sperm during fertilization, and the newly discovered sperm structure called the atypical centriole may contribute to infertility, miscarriages and birth defects. “Together, these studies call for a revision in our understanding of sperm centrioles both in sperm movement and in the early embryo,” Avidor-Reiss said. Avidor-Reiss believes this discovery can open the door to new possibilities to help families understand why they may be having trouble getting pregnant. If the head and tail of the sperm aren’t moving together, the sperm isn’t going to move efficiently enough to get to the egg. “If the centriole is defective, this coupling between the sperm tail and head is going to be defective,” Avidor-Reiss said. “In a patient when we don’t know what is wrong, potentially we can look at the way the sperm’s tail moves and reverse engineer it to determine centriole functionality to determine couple’s infertility.” He also said finding this movement can be used in the future to predict which sperm have a good centriole that can support life. “Right now, people don’t know what to fix,” Avidor-Reiss said. “We can pinpoint the problem. This knowledge allows us to identify a subgroup of infertile men that was not revealed before.” The new research shows that in the sperm of mammals there is a cascade of internal sliding formations in the neck’s atypical distal centriole, typical proximal centriole, and surrounding material that links tail beating with asymmetric head kinking. Using a STORM immunofluorescent microscope in the UToledo Instrumentation Center, the researchers were able to show the left and right side of the atypical centriole move about 300 nanometers relative to each other. Though it’s a small number, it marks dramatic movement in a cell considering the average protein diameter is five nanometers. Ph.D. student Luke Achinger, who recently graduated from UToledo with a bachelor’s degree in biology, sang bass in the University’s premier choral ensemble as an undergraduate and penned lyrics about his lab’s new discovery, explaining how the new movement works in a song called “Twitch, Roll and Yaw.” (Video below.) “We love to promote science and art, and in this case, we are showing that the sperm beats in unity. The head of the sperm is not isolated from the tail. The neck including the atypical and typical centrioles may act as a morphological computer, or sperm brain, that coordinates the sperm movement,” Avidor-Reiss said. “The song is a creative way to understand a big change. The centriole always looked the same over the last billion years. It’s one of most conservative structures in the cell. We found something different that functions in the opposite manner, evolving from a shock absorber to a transmission system.” Reference: “A dynamic basal complex modulates mammalian sperm movement” by Sushil Khanal, Miguel Ricardo Leung, Abigail Royfman, Emily L. Fishman, Barbara Saltzman, Hermes Bloomfield-Gadêlha, Tzviya Zeev-Ben-Mordehai and Tomer Avidor-Reiss, 21 June 2021, Nature Communications. DOI: 10.1038/s41467-021-24011-0 This study was an international collaboration with Dr. Tzviya Zeev-Ben-Mordehai’s lab at Utrecht University in the Netherlands, which performed state-of-the-art cryo-electron microscopy of the sperm neck, and Hermes Bloomfield-Gadêlha at the University of Bristol in the United Kingdom, who performed mathematical and waveform analysis. The NIRVANA field-test kit. Credit: Mo Li/KAUST The field test, called NIRVANA, can simultaneously detect and sequence SARS-CoV-2, influenza and other viruses. Clinicians using a new viral screening test can not only diagnose COVID-19 in a matter of minutes with a portable, pocket-sized machine, but can also simultaneously test for other viruses—like influenza—that might be mistaken for the coronavirus. At the same time, they can sequence the virus, providing valuable information on the spread of COVID-19 mutations and variants. The new test, dubbed NIRVANA, was described online on March 31, 2021, by a multi-institution team of scientists in the journal Med. “This is a virus detection and surveillance method that doesn’t require an expensive infrastructure like other approaches,” says Juan Carlos Izpisua Belmonte, co-corresponding author and a professor in Salk’s Gene Expression Laboratory. “We can accomplish with one portable test the same thing that others are using two or three different tests, with different machines, to do.” Around the world, more than 100 million people have been infected with SARS-CoV-2, the virus that causes COVID-19. A staggering 500,000 Americans have died from COVID-19 to date. Testing the population is key to stopping the spread of the virus. In addition, tracking the spread of new SARS-CoV-2 variants—some of which could respond differently to treatments or vaccines—is critical. Today, the standard approach to determining whether a nasal swab is positive for COVID-19 is to run a polymerase chain reaction (PCR) test to detect genetic material from the SARS-CoV-2 virus. If the sample is negative, however, patients and clinicians don’t get any information on what might be causing the coronavirus-like symptoms—unless they run separate PCR tests, using different swab samples, for other viruses. And if the sample is positive for SARS-CoV-2, they don’t learn which COVID-19 variant a patient is infected with unless another set of tests is run; those require a large and expensive next-generation gene-sequencing machine. From left: Juan Carlos Izpisua Belmonte and Mo Li. Credit: Juan Carlos Izpisua Belmonte Last summer, Mo Li, an assistant professor of bioscience at King Abdullah University of Science and Technology in Saudi Arabia, was pondering ways he could lend his expertise in genetic engineering and nanopore sequencing to combat the COVID-19 pandemic. Li, who previously spent six years as a Salk postdoctoral researcher in the Izpisua Belmonte lab, wondered whether a gene-detection approach called isothermal recombinase polymerase amplification (RPA) coupled with real-time nanopore sequencing might be more useful—and faster, cheaper and more portable—than the current COVID-19 testing approach. He teamed up with Izpisua Belmonte to find out. Unlike PCR, which cycles through lower and higher temperatures to separate DNA strands and copy them, RPA uses proteins—rather than temperature changes—to accomplish the same thing in only 20 minutes. The technology lets researchers copy longer stretches of DNA, and probe for multiple genes at the same time. Simultaneous Detection of Multiple Viruses “We quickly realized that we could use this technique to not only detect SARS-CoV-2, but other viruses at the same time,” says Li. In the new paper, Li and Izpisua Belmonte describe a small, portable device that can screen 96 samples at the same time using the RPA assay. They call the method NIRVANA, for “nanopore sequencing of isothermal rapid viral amplification for near real-time analysis.” The scientists designed NIRVANA to simultaneously test samples for COVID-19, influenza A, human adenovirus, and non-SARS-CoV-2 human coronavirus. In just 15 minutes, the researchers report, the device begins to report positive and negative results. And within three hours, the device finalizes results on all 96 samples—including the sequences of five regions of SARS-CoV-2 that are particularly prone to accumulate mutations leading to new variants such as the B.1.1.7 variant identified in the UK. Li and Izpisua Belmonte tested NIRVANA on 10 samples known to be positive for SARS-CoV-2, 60 samples of unknown SARS-CoV-2 status, as well as samples of municipal wastewater harboring the SARS-COV-2 virus and others. In all cases, the assay was able to correctly identify which viruses were present. The sequencing data also allowed them to narrow down the origin of SARS-CoV-2 in positive samples; differentiating strains from China and Europe, for instance. Flexible Design for Future Pathogens “The design of this assay is really flexible, so it’s not just limited to the examples we’ve shown,” says Li. “We can easily adapt it to tackle another pathogen, even something new and emergent.” With the small size and portability of the NIRVANA workflow, it could be used for fast virus detection at schools, airports, or ports, the researchers say. It also could be used to monitor wastewater or streams for the presence of new viruses. “The pandemic has provided two important lessons: first, test widely and quickly, and second, know your variants. Our NIRVANA method provides a promising solution to these two challenges not only for the current pandemic but also for possible future ones,” says Izpisua Belmonte, who holds the Roger Guillemin Chair at Salk. Market analysis would be required to determine whether the initial cost of commercialization—and the constant tweaks to the test needed to make sure it detected new variants or new viruses of interest—are worth it, Belmonte adds. Reference: “Simultaneous Detection and Mutation Surveillance of SARS-CoV-2 and co-infections of multiple respiratory viruses by Rapid field-deployable sequencing” by Chongwei Bi, Gerardo Ramos-Mandujano, Yeteng Tian, Sharif Hala, Jinna Xu, Sara Mfarrej, Concepcion Rodriguez Esteban, Estrella Nuñez Delicado, Fadwa S. Alofi, Asim Khogeer, Anwar M. Hashem, Naif A.M. Almontashiri, Arnab Pain, Juan Carlos Izpisua Belmonte and Mo Li, 31 March 2021, Med. DOI: 10.1016/j.medj.2021.03.015 In addition to Izpisua Belmonte and Li, other authors on the study were Concepcion Rodriguez Esteban of Salk; Chongwei Bi, Gerargo Ramos-Mandujano, Sharis Hala, Jinna Xu, Sara Mfarrej, Yeteng Tian and Arnab Pain of King Abdullah University of Science and Technology (KAUST); Estrella Nunez Delicado of UCAM Universidad Católica San Antonio de Murcia; Fadwa Alofi of King Fahad Hospital; Asim Khogeer of Saudi Arabia’s Ministry of Health; Anwar Hashem of King Abdulaziz University; and Naif Almontashiri of Taibah University. The work described in the current paper was supported by a competitive research grant from the King Abdullah University of Science and Technology. RRG455KLJIEVEWWF |
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