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一頭牛日式燒肉包廂適合尾牙嗎?》台中公益路餐廳排行榜|10家熱門店家解析 |
| 在地生活|雲嘉南 2026/04/20 04:36:07 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人,臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」,從精緻西餐到創意火鍋,從日式丼飯到義式早午餐,每走幾步,就會有完全不同的特色料理餐廳。 這次我特別花了一整個月,實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店,也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準,整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你,找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型,從高質感牛排館到巷弄系早午餐,每一間都有自己獨特的風格。為了讓整體比較更客觀,我依照以下四大面向進行評比,並搭配實際用餐體驗來打分。
整體而言,我希望這份評比不只是「哪家好吃」,而是幫你在不同情境下(約會、家庭聚餐、朋友小聚、商業午餐)都能快速找到合適的選擇。畢竟,美食不只是味覺的滿足,更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段,從火鍋、燒肉到中式料理與早午餐,每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單,橫跨平價、創意、高級各路風格。
一頭牛日式燒肉|炭香濃郁的和牛饗宴,約會聚餐首選
走在公益路上,很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面,搭配昏黃燈光與暖色調的內裝,讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大,但桌距規劃得宜,每桌皆設有獨立排煙設備,烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂,絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網,讓人完全不用分心。整場用餐過程就像一場表演,從視覺、嗅覺到味覺都被滿足。 綜合評分
地址:408臺中市南屯區公益路二段162號電話:04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」,更像是一場五感盛宴。從進門那一刻到最後一道甜點,都能感受到他們對細節的用心。 TANG Zhan 湯棧|文青系火鍋代表,麻香湯底與視覺美感並重
在公益路這條美食戰線上,TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址:408臺中市南屯區公益路二段248號電話:04-22580617 官網:https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度,又結合現代風格與細節服務,讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店,湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店|明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂,那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主,陽光灑進室內,讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧,建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味,選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口,米芯保留微Q口感;而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香,口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹,無論是一個人帶電腦工作、或朋友聚餐,都能找到舒服角落。餐點上桌速度穩定,服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」,很適合想遠離忙碌日常的人。 綜合評分
地址:40861臺中市南屯區公益路二段18號電話:04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇,而是以「剛剛好」的份量與風味,陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店,NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物|平價卻用心的湯頭系火鍋,家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上,加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐,但靠著實在的湯頭與親切的服務,默默吸引許多回頭客。每到用餐時間,總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底,湯頭清澈卻不單薄,越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向,桌距寬敞、座位舒適,帶小孩來也不覺擁擠。店員態度親切,補湯、收盤都很勤快,給人一種「被照顧著」的安心感。 綜合評分
地址:403臺中市西區公益路288號電話:0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤,而是用最簡單的湯頭與新鮮食材,傳遞出家常卻不平凡的溫度。 印月餐廳|中式料理的藝術演繹,宴客與家庭聚會首選
說到臺中公益路的中式料理代表,印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名,把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設,每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏,都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法,讓人感受到「被款待」的尊榮感。 綜合評分
地址:408臺中市南屯區公益路二段818號電話:0422511155 小結語印月餐廳是一間「不只吃飯,更像品味生活」的地方。 KoDō 和牛燒肉|極致職人精神,專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗,一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉,以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址:403臺中市西區公益路260號電話:0423220312 官網:https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳,而是一場體驗。 永心鳳茶|在茶香裡用餐的優雅時光,臺味早午餐的新詮釋
走進 永心鳳茶公益店,彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤,無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」,都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和,對茶品介紹詳盡。上餐節奏剛好,不急不徐。 綜合評分
地址:40360臺中市西區公益路68號三樓(勤美誠品)電話:0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓|老饕級江浙功夫菜,穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主,兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址:408臺中市南屯區公益路二段95號電話:0423202322 官網:https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司|低調奢華的無菜單日料,職人手藝詮釋旬味極致
在熱鬧的公益路上,一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase(無菜單料理) 形式,每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘,節奏緩慢但沉穩。 綜合評分
地址:408臺中市南屯區公益路二段25號電話:0423206368 官網:https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂|人氣爆棚的和牛燒肉聖地,肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳,茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐,價格約落在 $700–$1000 間,份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快,整場用餐流程流暢無壓力。 綜合評分
地址:403臺中市西區公益路268號電話:0423281167 官網:https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質+輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後,臺中公益路不只是一條美食街,而是一段生活風景線。 有的餐廳講究細膩與儀式感,像 一頭牛日式燒肉 與 一笈壽司,讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心,像 加分昆布鍋物、永心鳳茶,讓人明白吃飯不只是為了飽足,而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店,則用穩定的品質與熱絡的氛圍,成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店,構成了公益路最動人的縮影 有華麗的,也有溫柔的;有傳統的,也有創新的。 每一家都在自己的風格裡發光,讓人吃到的不只是料理,而是一種生活的溫度與節奏。 對我而言,這不僅是一場美食旅程,更是一趟關於「臺中味道」的回憶之旅。 FAQ:關於臺中公益路美食常見問題Q1:公益路哪一區的餐廳最集中? Q2:需要提前訂位嗎? 最後的話若要用一句話形容這趟美食之旅,我會說: TANG Zhan 湯棧食材新鮮嗎? 如果你也和我一樣喜歡用味蕾探索一座城市,那就把這篇公益路美食攻略收藏起來吧。TANG Zhan 湯棧家庭過節聚會適合嗎? 無論是約會、慶生、家庭聚餐,或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。KoDō 和牛燒肉上餐速度快嗎? 下一餐,不妨從這10家開始。TANG Zhan 湯棧CP 值高嗎? 打開手機、約上朋友,讓公益路成為你生活裡最容易抵達的小確幸。印月餐廳春酒場面夠體面嗎? 如果你有私心愛店,也歡迎留言分享,印月餐廳員工聚會夠氣派嗎? 你的推薦,可能讓我下一趟美食旅程變得更精彩。一頭牛日式燒肉團體宴客合適嗎? Cuckoo finch eggs laid by different females. Diversity of maternally inherited egg phenotypes within a single interbreeding species, the brood-parasitic cuckoo finch. Different cuckoo finch matrilines mimic the eggs of different host species (here, tawny-flanked prinia and red-faced cisticola) and have further diversified to approximate the range of variable egg “signatures” within each host species, an anti-parasite adaptation that aids host parents in recognizing their own eggs. Credit: Claire N. Spottiswoode While many people around the world just finished unwrapping their Easter eggs, scientists have solved one of nature’s biggest criminal cases, an egg forgery scandal two million years in the making. Their findings suggest that the victims of this fraud may now be gaining the upper hand. Many birds around the world side-step the costs of parenthood by laying their eggs in the nest of other species. Known as “brood parasitism,” this way of life has many advantages but also presents challenges such as how to convince the other species to accept a foreign egg. Many brood parasites achieve this by mimicking the colors and patterns of their host’s eggs, but some exploit the care of several different host species whose eggs all look different. Cuckoo finch egg in zitting cisticola nest. Cuckoo finch eggs closely mimic the color and pattern of the eggs of each of their several host species, to trick host parents into accepting the parasitic egg as one of their own. Here a cuckoo finch has successfully had its egg (at left) accepted in the nest of a zitting cisticola (egg at right). Credit: Claire N. Spottiswoode How can a single brood-parasitic bird species imitate the eggs of multiple different bird species at the same time in order to trick them into raising their young? And how do these parasitic forgers pass this capability on to their young despite interbreeding between birds raised by different hosts? These questions have been puzzling scientists for more than a century. Now genetic research by an international team led by Professor Claire Spottiswoode from the Cambridge’s Department of Zoology and the FitzPatrick Institute of African Ornithology, University of Cape Town; and Professor Michael Sorenson at Boston University, has made a major breakthrough, and their findings may be bad news for the egg forgers. The study, published on April 11, 2022, in Proceedings of the National Academy of Sciences (PNAS), focuses on the genetics of egg mimicry in the cuckoo finch, a species that adopts a brood-parasitic lifestyle and exploits many species of warbler across Africa. A tawny-flanked prinia, a common host species of the cuckoo finch, captured in Zambia for genetic sampling with the help of field assistant Tom Hamusikili. Credit: Claire N. Spottiswoode Female cuckoo finches inherit their capacity to imitate the look of their hosts’ eggs from their mothers, according to the research study, via the female-specific W chromosome (analogous to the male-specific Y chromosome in humans). Such ‘maternal inheritance’ allows cuckoo finches to side-step the risk of inheriting the wrong mimicry genes from a father raised by a different host, and so has allowed distinct lineages of cuckoo finch females to evolve specialized egg mimicry of several different host species. Such mimicry dupes host parents into accepting a parasitic egg as their own rather than throwing it out of the nest, and so has been crucial to the success of these African birds. But the researchers believe that this long-established ‘genetic architecture’ of maternal inheritance may come back to haunt the cuckoo finches. Dr. Spottiswoode said: “In this particular coevolutionary arms race between species, natural selection has created a double-edged sword.” “While maternal inheritance has allowed cuckoo finches to exploit multiple host species, it’s likely to slow their ability to evolve counter-adaptations as their hosts evolve new defenses. “In particular, parasites face a daunting challenge because some host species have in return evolved an astonishing diversity of egg color and pattern ‘signatures’, that help hosts to distinguish their own eggs from parasitic mimics.” Cuckoo finch mimicry of tawny-flanked prinia eggs This photograph shows eggs of the cuckoo finch (middle circle) and one of its common host species, the tawny-flanked prinia (outer circle), revealing the diversity of host egg color and pattern “signatures” (an anti-parasite adaptation that aids host parents in recognizing their own eggs) that are mimicked by cuckoo finch egg “forgeries.” The eggs of the cuckoo finch (middle circle) and a common host species, the tawny-flanked prinia (outer circle), are shown in this photograph, revealing the diversity of host egg color and pattern “signatures” that are mimicked by cuckoo finch egg “forgeries.” Credit: Claire N. Spottiswoode However, cuckoo finches do not mimic the rich olive-green eggs (top left) laid by some tawny-flanked prinia females. The new study suggests that a genetic constraint in cuckoo finches may account for their apparent inability to mimic this host egg type. The field data were collected at a study site in southern Zambia together with Dr. Wenfei Tong and Dr. Gabriel Jamie from the University of Cambridge and Ailsa Green, Silky Hamama, Ian Taylor, and Collins Moya from the surrounding community in Zambia. Cuckoo finches in this area trick four different species of grass-warbler to devastating effect: if host parents fail to detect and remove a parasitic egg in their nest, the young cuckoo finch typically outcompetes the hosts’ own hatchlings, which soon starve to death. Cuckoo finch and host chicks. Brood parasitism is costly for hosts because a cuckoo finch chick begs for food very vigorously as soon as it hatches, outcompeting the host parents’ own chicks (here zitting cisticolas) which typically soon die of starvation. Credit: Claire N. Spottiswoode The team collected DNA samples from 196 cuckoo finches from 141 nests belonging to the four grass-warbler species and studied the majority by sequencing thousands of short segments across their genomes. In their fightback against the forgers, grass-warblers have become skilled quality controllers, rejecting eggs that differ from their own in color and pattern, and all four species have evolved the ability to deposit unique ‘signatures’ onto their own eggs to enhance their detection of intruders. Tawny-flanked prinias, for example, lay eggs with blue, white, red, or olive-green backgrounds overlaid with a variety of patterns. Cuckoo finches have responded not only by evolving mimicry of the eggs of their several host species, but have also further diversified to mimic at least some of the signature-like variation seen in the eggs of different females within each host species. The team established that both abilities are handed down through maternal inheritance, finally validating a hypothesis first proposed in 1933 by ornithologists pondering how the common cuckoo in Europe was similarly able to mimic the eggs of several different host species. Forgers facing an uncertain future? The researchers believe that the cuckoo finches now face an uphill struggle because they cannot recombine the different forgery traits evolved by their separate family lines. For example, two different lineages of cuckoo finch mothers have evolved eggs with either blue or red backgrounds, as an evolutionary response to similar diversity in their tawny-flanked prinia hosts, but there is no evidence that they can create the precise mixture of pigments needed to produce the olive-green eggs that some host females can produce. Study co-author Collins Moya (left) carrying out fieldwork in the grasslands of southern Zambia, together with field assistant Kiverness Moono (right). Credit: Claire N. Spottiswoode In a previous study, Professor Spottiswoode found that a growing proportion of eggs laid by tawny-flanked prinia hosts are olive-green, suggesting this is part of an accelerating evolutionary fightback. As expected, the team found that these host birds are passing down their anti-fraud ‘egg signature’ abilities through a different genetic process (bi-parental inheritance) to that used by the cuckoo finches. Spottiswoode said: “Cuckoo finches are missing out on a powerful source of evolutionary novelty and that could prove costly in this ongoing arms race.” She added: “The way they inherit their ability to mimic host eggs has a downside by likely making the grass-warblers’ defenses more effective, and constraining the parasite’s ability to respond. “We may see the emergence of unforgeable egg signatures which could force cuckoo finches to switch to other naïve host species. Or the parasitic birds might become increasingly dependent on young host individuals that haven’t yet learned their own signatures and are bad at spotting mismatched eggs.” The study argues that ‘selection from host defenses drove cuckoo finches to transfer control of egg appearance to the maternally inherited part of the genome’ at least 2 million years ago. Reference: “Genetic architecture facilitates then constrains adaptation in a host–parasite coevolutionary arms race” by Claire N. Spottiswoode, Wenfei Tong, Gabriel A. Jamie, Katherine F. Stryjewski, Jeffrey M. DaCosta, Evan R. Kuras, Ailsa Green, Silky Hamama, Ian G. Taylor, Collins Moya, and Michael D. Sorenson, 11 April 2022, Proceedings of the National Academy of Sciences. DOI: 10.1073/pnas.2121752119 The research was funded primarily by fellowships from the Biotechnology and Biological Sciences Research Council and the Royal Society, and by the National Science Foundation. An immune signal promotes the production of energy-burning beige fat, potentially leading to new ways to treat obesity and metabolic disorders. Cytokine increases production of “beige fat” to burn more cellular energy. An immune signal promotes the production of energy-burning “beige fat,” according to a new study published in the open-access journal PLOS Biology by Zhonghan Yang of Sun Yat-Sen University, Guangzhou, China, and colleagues. The finding may lead to new ways to reduce obesity and treat metabolic disorders. The beige color in beige fat comes from its high concentration of mitochondria, the cell’s powerhouses. Mitochondria burn high-energy molecules like fats and sugars with oxygen, releasing energy. Normally, that energy is stored as ATP, the energy currency that the cell uses for almost all its activities. But in beige fat, mitochondria accumulate a protein called “uncoupling protein-1” that limits ATP production, generating heat instead. Babies are born with “brown fat,” a similar tissue concentrated in the shoulder region, which helps them stay warm, but brown fat is gradually lost with age. Not-so-beige fat is more widely distributed and can be generated throughout life in response to both cold and neuronal or hormonal stimulation. Recent work, including by the authors of the new study, has revealed that cytokines—immune system signaling molecules—play a role in regulation of beige fat. To explore that regulation further, the authors manipulated levels of the cytokine interleukin-25, and showed that an increase in the cytokine could mimic the effects of both cold and stimulation of a hormone receptor in increasing the production of beige fat in mice. They traced the signaling chain further, showing that IL-25 exerted its effects through two other cytokines, which in turn regulated immune cells called macrophages. Those cells acted on neurons that terminate in the beige fat tissue, promoting an increase in production of the neurotransmitter norepinephrine, which was already known to promote beige fat production. Thus, the authors’ work revealed the sequence of regulatory signals that begins with IL-25 and ends with release of norepinephrine and an increase in beige fat. Finally, the authors showed that administering IL-25 to mice that were eating a high-fat diet prevented them from becoming obese and improved their ability to maintain their responsiveness to insulin, which is impaired in chronic obesity. “Our results show that interleukin-25 plays a key role in production of beige fat,” Yang said, “and point toward increasing interleukin-25 signaling as a potential treatment for obesity.” Reference: “IL-25–induced shifts in macrophage polarization promote development of beige fat and improve metabolic homeostasis in mice” by Lingyi Li, Lei Ma, Zewei Zhao, Shiya Luo, Baoyong Gong, Jin Li, Juan Feng, Hui Zhang, Weiwei Qi, Ti Zhou, Xia Yang, Guoquan Gao and Zhonghan Yang, 5 August 2021, PLOS Biology. DOI: 10.1371/journal.pbio.3001348 Funding: This work was funded by the National Nature Science Foundation of China(grant number No: 81570764, 81770808, 81701414, 81872165 and 81871211), National Key R&D Program of China (grant number No. 2018YFA0800403), Guangdong Provincial Key R&D Program (grant number No: 2018B030337001 and 2019B020227003), Key Project of Nature Science Foundation of Guangdong Province, China (grant number No. 2019B1515120077), Guangdong Natural Science Fund (grant number No: 2019A1515011810 and 2020A1515010365), Guangdong Science and Technology Project (grant number No. 2017A020215075), Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translation Research of Hakka Population (grant number No. 2018B030322003KF01), Guangzhou Science and Technology Project (grant number No: 201807010069, 201803010017 and 202002020022) and Shenzhen Science and Technology Project (grant number No. JCYJ20190807154205627) received by Weiwei Qi, Ti Zhou, Xia Yang, Guoquan Gao and Zhonghan Yang. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Salk Institute scientists are using an AI software, SLEAP, to develop plants with enhanced root systems that can capture and store more carbon, aligning with global efforts to combat climate change. This tool has significantly improved the efficiency and accuracy of plant phenotype and genotype analysis, speeding up the creation of effective carbon-sequestering plants. Credit: Salk Institute A unique partnership at Salk leverages the deep learning software known as SLEAP to study plant characteristics, speeding up the development of plants that can combat climate change. The Intergovernmental Panel on Climate Change (IPCC) has stated that carbon removal is crucial for combating climate change and keeping global temperature increases in check. In alignment with this, scientists at Salk are leveraging the natural capacity of plants to absorb carbon dioxide by enhancing their root systems. This optimization aims to increase the amount of carbon stored and extend the duration of its storage. To design these climate-saving plants, scientists in Salk’s Harnessing Plants Initiative are using a sophisticated new research tool called SLEAP—an easy-to-use artificial intelligence (AI) software that tracks multiple features of root growth. Created by Salk Fellow Talmo Pereira, SLEAP was initially designed to track animal movement in the lab. Now, Pereira has teamed up with plant scientist and Salk colleague Professor Wolfgang Busch to apply SLEAP to plants. SLEAP and sleap-roots predict how the different parts of plant roots connect to each other by analyzing the geometry of the roots. Credit: Salk Institute Advanced Research with SLEAP In a study published in Plant Phenomics, Busch and Pereira debut a new protocol for using SLEAP to analyze plant root phenotypes—how deep and wide they grow, how massive their root systems become, and other physical qualities that, prior to SLEAP, were tedious to measure. The application of SLEAP to plants has already enabled researchers to establish the most extensive catalog of plant root system phenotypes to date. What’s more, tracking these physical root system characteristics helps scientists find genes affiliated with those characteristics, as well as whether multiple root characteristics are determined by the same genes or independently. This allows the Salk team to determine what genes are most beneficial to their plant designs. “This collaboration is truly a testament to what makes Salk science so special and impactful,” says Pereira. “We’re not just ‘borrowing’ from different disciplines—we’re really putting them on equal footing in order to create something greater than the sum of its parts.” From left: Talmo Pereira, Elizabeth Berrigan, and Wolfgang Busch. Credit: Salk Institute Prior to using SLEAP, tracking the physical characteristics of both plants and animals required a lot of labor that slowed the scientific process. If researchers wanted to analyze an image of a plant, they would need to manually flag the parts of the image that were and weren’t plant—frame-by-frame, part-by-part, pixel-by-pixel. Only then could older AI models be applied to process the image and gather data about the plant’s structure. What sets SLEAP apart is its unique use of both computer vision (the ability for computers to understand images) and deep learning (an AI approach for training a computer to learn and work like the human brain). This combination allows researchers to process images without moving pixel-by-pixel, instead skipping this intermediate labor-intensive step to jump straight from image input to defined plant features. “We created a robust protocol validated in multiple plant types that cuts down on analysis time and human error, while emphasizing accessibility and ease-of-use—and it required no changes to the actual SLEAP software,” says first author Elizabeth Berrigan, a bioinformatics analyst in Busch’s lab. Impact of SLEAP on Plant Breeding Without modifying the baseline technology of SLEAP, the researchers developed a downloadable toolkit for SLEAP called sleap-roots (available as open-source software here). With sleap-roots, SLEAP can process biological traits of root systems like depth, mass, and angle of growth. The Salk team tested the sleap-roots package in a variety of plants, including crop plants like soybeans, rice, and canola, as well as the model plant species Arabidopsis thaliana—a flowering weed in the mustard family. Across the variety of plants trialed, they found the novel SLEAP-based method outperformed existing practices by annotating 1.5 times faster, training the AI model 10 times faster, and predicting plant structure on new data 10 times faster, all with the same or better accuracy than before. Together with massive genome sequencing efforts for elucidating the genotype data in large numbers of crop varieties, these phenotypic data, such as a plant’s root system growing especially deep in soil, can be extrapolated to understand the genes responsible for creating that especially deep root system. SLEAP and sleap-roots automatically detect landmarks across the entire root system architecture. Credit: Salk Institute This step—connecting phenotype and genotype—is crucial in Salk’s mission to create plants that hold on to more carbon and for longer, as those plants will need root systems designed to be deeper and more robust. Implementing this accurate and efficient software will allow the Harnessing Plants Initiative to connect desirable phenotypes to targetable genes with groundbreaking ease and speed. “We have already been able to create the most extensive catalog of plant root system phenotypes to date, which is really accelerating our research to create carbon-capturing plants that fight climate change,” says Busch, the Hess Chair in Plant Science at Salk. “SLEAP has been so easy to apply and use, thanks to Talmo’s professional software design, and it’s going to be an indispensable tool in my lab moving forward.” Accessibility and reproducibility were at the forefront of Pereira’s mind when creating both SLEAP and sleap-roots. Because the software and sleap-roots toolkit are free to use, the researchers are excited to see how sleap-roots will be used around the world. Already, they have begun discussions with NASA scientists hoping to utilize the tool not only to help guide carbon-sequestering plants on Earth, but also to study plants in space. At Salk, the collaborative team is not yet ready to disband—they are already embarking on a new challenge of analyzing 3D data with SLEAP. Efforts to refine, expand, and share SLEAP and sleap-roots will continue for years to come, but its use in Salk’s Harnessing Plants Initiative is already accelerating plant designs and helping the Institute make an impact on climate change. Reference: “Fast and Efficient Root Phenotyping via Pose Estimation” by Elizabeth M. Berrigan, Lin Wang, Hannah Carrillo, Kimberly Echegoyen, Mikayla Kappes, Jorge Torres, Angel Ai-Perreira, Erica McCoy, Emily Shane, Charles D. Copeland, Lauren Ragel, Charidimos Georgousakis, Sanghwa Lee, Dawn Reynolds, Avery Talgo, Juan Gonzalez, Ling Zhang, Ashish B. Rajurkar, Michel Ruiz, Erin Daniels, Liezl Maree, Shree Pariyar, Wolfgang Busch and Talmo D. Pereira, 12 April 2024, Plant Phenomics. DOI: 10.34133/plantphenomics.0175 Other authors include Lin Wang, Hannah Carrillo, Kimberly Echegoyen, Mikayla Kappes, Jorge Torres, Angel Ai-Perreira, Erica McCoy, Emily Shane, Charles Copeland, Lauren Ragel, Charidimos Georgousakis, Sanghwa Lee, Dawn Reynolds, Avery Talgo, Juan Gonzalez, Ling Zhang, Ashish Rajurkar, Michel Ruiz, Erin Daniels, Liezl Maree, and Shree Pariyar of Salk. The work was supported by the Bezos Earth Fund, the Hess Corporation, the TED Audacious Project, and the National Institutes of Health (RF1MH132653). RRG455KLJIEVEWWF |
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