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文章數：76 |
 茶六燒肉堂尾牙氣氛熱鬧嗎？》台中公益路高分美食推薦｜10間絕對不踩雷 |
| 創作｜散文 2026/04/20 12:46:24 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人，臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」，從精緻西餐到創意火鍋，從日式丼飯到義式早午餐，每走幾步，就會有完全不同的特色料理餐廳。 這次我特別花了一整個月，實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店，也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準，整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你，找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型，從高質感牛排館到巷弄系早午餐，每一間都有自己獨特的風格。為了讓整體比較更客觀，我依照以下四大面向進行評比，並搭配實際用餐體驗來打分。
整體而言，我希望這份評比不只是「哪家好吃」，而是幫你在不同情境下（約會、家庭聚餐、朋友小聚、商業午餐）都能快速找到合適的選擇。畢竟，美食不只是味覺的滿足，更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段，從火鍋、燒肉到中式料理與早午餐，每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單，橫跨平價、創意、高級各路風格。
一頭牛日式燒肉｜炭香濃郁的和牛饗宴，約會聚餐首選
走在公益路上，很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面，搭配昏黃燈光與暖色調的內裝，讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大，但桌距規劃得宜，每桌皆設有獨立排煙設備，烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂，絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網，讓人完全不用分心。整場用餐過程就像一場表演，從視覺、嗅覺到味覺都被滿足。 綜合評分
地址：408臺中市南屯區公益路二段162號電話：04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」，更像是一場五感盛宴。從進門那一刻到最後一道甜點，都能感受到他們對細節的用心。 TANG Zhan 湯棧｜文青系火鍋代表，麻香湯底與視覺美感並重
在公益路這條美食戰線上，TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址：408臺中市南屯區公益路二段248號電話：04-22580617 官網：https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度，又結合現代風格與細節服務，讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店，湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店｜明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂，那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主，陽光灑進室內，讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧，建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味，選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口，米芯保留微Q口感；而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香，口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹，無論是一個人帶電腦工作、或朋友聚餐，都能找到舒服角落。餐點上桌速度穩定，服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」，很適合想遠離忙碌日常的人。 綜合評分
地址：40861臺中市南屯區公益路二段18號電話：04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇，而是以「剛剛好」的份量與風味，陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店，NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物｜平價卻用心的湯頭系火鍋，家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上，加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐，但靠著實在的湯頭與親切的服務，默默吸引許多回頭客。每到用餐時間，總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底，湯頭清澈卻不單薄，越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向，桌距寬敞、座位舒適，帶小孩來也不覺擁擠。店員態度親切，補湯、收盤都很勤快，給人一種「被照顧著」的安心感。 綜合評分
地址：403臺中市西區公益路288號電話：0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤，而是用最簡單的湯頭與新鮮食材，傳遞出家常卻不平凡的溫度。 印月餐廳｜中式料理的藝術演繹，宴客與家庭聚會首選
說到臺中公益路的中式料理代表，印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名，把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設，每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏，都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法，讓人感受到「被款待」的尊榮感。 綜合評分
地址：408臺中市南屯區公益路二段818號電話：0422511155 小結語印月餐廳是一間「不只吃飯，更像品味生活」的地方。 KoDō 和牛燒肉｜極致職人精神，專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗，一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉，以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址：403臺中市西區公益路260號電話：0423220312 官網：https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳，而是一場體驗。 永心鳳茶｜在茶香裡用餐的優雅時光，臺味早午餐的新詮釋
走進 永心鳳茶公益店，彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤，無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」，都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和，對茶品介紹詳盡。上餐節奏剛好，不急不徐。 綜合評分
地址：40360臺中市西區公益路68號三樓(勤美誠品)電話：0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓｜老饕級江浙功夫菜，穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主，兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址：408臺中市南屯區公益路二段95號電話：0423202322 官網：https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司｜低調奢華的無菜單日料，職人手藝詮釋旬味極致
在熱鬧的公益路上，一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase（無菜單料理） 形式，每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘，節奏緩慢但沉穩。 綜合評分
地址：408臺中市南屯區公益路二段25號電話：0423206368 官網：https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂｜人氣爆棚的和牛燒肉聖地，肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳，茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐，價格約落在 $700–$1000 間，份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快，整場用餐流程流暢無壓力。 綜合評分
地址：403臺中市西區公益路268號電話：0423281167 官網：https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質＋輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後，臺中公益路不只是一條美食街，而是一段生活風景線。 有的餐廳講究細膩與儀式感，像 一頭牛日式燒肉 與 一笈壽司，讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心，像 加分昆布鍋物、永心鳳茶，讓人明白吃飯不只是為了飽足，而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店，則用穩定的品質與熱絡的氛圍，成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店，構成了公益路最動人的縮影 有華麗的，也有溫柔的；有傳統的，也有創新的。 每一家都在自己的風格裡發光，讓人吃到的不只是料理，而是一種生活的溫度與節奏。 對我而言，這不僅是一場美食旅程，更是一趟關於「臺中味道」的回憶之旅。 FAQ：關於臺中公益路美食常見問題Q1：公益路哪一區的餐廳最集中？ Q2：需要提前訂位嗎？ 最後的話若要用一句話形容這趟美食之旅，我會說： 三希樓份量足夠嗎？ 如果你也和我一樣喜歡用味蕾探索一座城市，那就把這篇公益路美食攻略收藏起來吧。三希樓飲料值得加點嗎？ 無論是約會、慶生、家庭聚餐，或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。TANG Zhan 湯棧真的有那麼好吃嗎？ 下一餐，不妨從這10家開始。印月餐廳食材新鮮嗎？ 打開手機、約上朋友，讓公益路成為你生活裡最容易抵達的小確幸。一笈壽司長官聚餐合適嗎？ 如果你有私心愛店，也歡迎留言分享，永心鳳茶情侶來合適嗎？ 你的推薦，可能讓我下一趟美食旅程變得更精彩。三希樓適合聚餐嗎？ Antarctic Penguins A new study reveals that proposed Marine Protected Areas (MPAs) in the Southern Ocean could vastly increase protection for key penguin habitats. New research led by BirdLife International, the University of East Anglia (UEA) and British Antarctic Survey highlights how a proposed network of marine protected areas could help safeguard some of the most important areas at sea for breeding Antarctic penguins. The findings, published today in the journal Frontiers in Marine Science, show that if all the Marine Protected Areas (MPAs) proposed around Antarctica were adopted, the permanent conservation of high-quality areas for a flagship group of Antarctic wildlife — the penguins — would increase by between 49% and 100% depending on the species. Adélie penguins on the Antarctic Penisula. Credit: Heather Lynch The Southern Ocean surrounding Antarctica is home to thousands of unique species, including seals, whales and four species of penguins — the Adélie, Chinstrap, Gentoo, and Emperor. Many of these feed on krill, tiny shrimp-like crustaceans, which are also the target of large commercial fisheries, who harvest them for a variety of krill-based products including fish food. Penguins as Environmental Indicators Penguins are often considered an indicator species whose populations reflect the state of the surrounding marine environment. However, many vital penguin habitats remain unprotected, leaving them susceptible to human-related threats such as pollution, overfishing and climate change. Gentoo penguins in the Antarctic Peninsula, where a Marine Protected Area has been proposed. Credit: Derek Oyen The study used a new approach based on colony location, population estimates, and tracking data, to identify globally important areas for penguin species around Antarctica, pinpointing 63 key sites. Known as Important Bird and Biodiversity Areas (IBAs), they are used by at least 1% of a species’ global population. They represent important foraging grounds, surrounding breeding colonies of several thousands of individuals when penguins congregate to raise their chicks. Adopted MPAs, proposed MPAs and MPA planning area (See Appendix–Study area and key spatial data for additional details) within the study area (CCAMLR MPA planning domains (D) 1, 3, 4, 7, 8, 9), and overlap with identified network of 63 marine IBAs for penguin species. Text black circles adjacent to marine IBAs indicate species present within the marine IBA. Adopted MPAs within the planning domains include, the Ross Sea Region MPA and the South Orkney Islands Southern Shelf MPA (SOISS MPA). Proposed MPAs include the Domain 1 MPA (D1MPA), Weddell Sea MPA Phase 1 (WSMPA Phase 1) and the East Antarctica MPA (EAMPA). Also shown are the Weddell Sea MPA Phase 2 Planning Area (WSMPA Phase 2) and the Voluntary Restricted Zones (VRZs) in which a majority of krill fishing fleets have limited their operations in certain regions during parts of the summer (AP: Antarctic Peninsula: 1 Oct – 1 Feb, GS: Gerlache Strait: 15 Oct – 15 Feb, SSI: South Shetland Islands: 1 Nov – 1 Mar). Inset map area indicated by black bounding box. The international team also examined krill fishery activities over the last 50 years and found that while its range of operation has contracted, a consistently disproportionate amount of krill is being harvested within the globally important areas for penguins compared to the total area in which the fishery operates. The results align with other studies which show that krill fisheries might be directly competing with penguins for crucial foraging resources. “Our findings provide critical evidence about the location and relevance of some of the most important areas globally for chick-rearing adult penguins breeding in Antarctica and nearby islands,” said lead author Dr Jonathan Handley, of Birdlife International. Adélie penguins on the Antarctic Penisula. Credit: Heather Lynch “Over the past five decades, krill fisheries have concentrated into a small number of areas in Antarctic waters, some of which we identified as important penguin foraging grounds. This poses a likely threat for several penguin colonies, especially when they are rearing chicks.” To control the increasing commercial interest in Antarctic fisheries and particularly krill resources, an international convention was established in 1982, governed by the Commission for the Conservation of Antarctic Marine Living Resources (CCAMLR) — an international convention comprised of 25 Member states, together with the European Union. Monitoring a Chinstrap Penguin Colony in Antarctica. Credit: Christian Aslund The convention envisioned the creation of a network of MPAs around Antarctica since 2002, but since 2016 only two have been implemented. Three more have been under discussion for several years but members have not been able to agree on their formal designation. Proposed MPAs Could Secure Penguin Futures Co-author Dr Aldina Franco, of UEA’s School of Environmental Sciences, said the new study supports the adoption of the proposed MPA network: “Recent studies have shown that krill fisheries could be directly competing with penguins for critical food supplies. The proposed Marine Protected Area network, which has recognized go/no go areas for krill fisheries, can help guarantee that enough krill is available for penguins.” Adélie penguins on the Antarctic Penisula. Credit: Heather Lynch Marie-Morgane Rouyer, who jointly led the research while a Masters student at UEA, added: “Marine resources need to be managed in a sustainable way if we are to guarantee the existence of these emblematic penguin species in the future.” The researchers identified new IBAs that are important for the conservation of Antarctic penguins and examined the overlap with existing and proposed MPAs. They found that Adélie and Emperor penguins currently have 27-31% of the important areas within adopted MPAs, but no Gentoo’s IBAs and only 1% of Chinstrap’s are within them. If all proposed MPAs for Antarctica are adopted then an average of 80% of the important areas for penguin conservation would be within an MPA. This highlights the importance of the proposed network, which ultimately could benefit not only Antarctic penguins, but some of the most unique wildlife on Earth. Reference: “Marine Important Bird and Biodiversity Areas for penguins in Antarctica, targets for conservation action” by Jonathan Handley, Marie-Morgane Rouyer, Elizabeth J Pearmain, Victoria Warwick-Evans, Katharina Teschke, Jefferson Hinke, Heather Lynch, Louise Emmerson, Colin Southwell, Gary Griffith, Cesar A Cardenas, Aldina M Franco, Philip Trathan and Maria P Dias, 20 January 2021, Frontiers in Marine Science. DOI: 10.3389/fmars.2020.602972 The research was funded by the Pew Charitable Trusts. A new study in Nature Biotechnology reveals that 4.5 million gamers have significantly advanced medical research by playing a mini-game within Borderlands 3, which helped map the evolutionary history of microbes in the human gut. This effort, led by McGill University and supported by multiple organizations, has improved existing algorithms and laid the groundwork for future AI tools, showcasing a unique synergy between video gaming and scientific research. Credit: Gearbox Major worldwide citizen science initiative advances understanding of the human microbiome. According to a recent study in Nature Biotechnology, leveraging gamers and video game technology can dramatically boost scientific research. 4.5 million gamers around the world have advanced medical science by helping to reconstruct microbial evolutionary histories using a minigame included inside the critically and commercially successful video game, Borderlands 3. Their playing has led to a significantly refined estimate of the relationships of microbes in the human gut. The results of this collaboration will both substantially advance our knowledge of the microbiome and improve on the AI programs that will be used to carry out this work in the future. Tracing the evolutionary relationships of bacteria By playing Borderlands Science, a mini-game within the looter-shooter video game Borderlands 3, these players have helped trace the evolutionary relationships of more than a million different kinds of bacteria that live in the human gut, some of which play a crucial role in our health. This information represents an exponential increase in what we have discovered about the microbiome up till now. By aligning rows of tiles which represent the genetic building blocks of different microbes, humans have been able to take on tasks that even the best existing computer algorithms have been unable to solve yet. The project was led by McGill University researchers, developed in collaboration with Gearbox Entertainment Company, an award-winning interactive entertainment company, and Massively Multiplayer Online Science (MMOS), a Swiss IT company connecting scientists to video games), and supported by the expertise and genomic material from the Microsetta Initiative led by Rob Knight from the Departments of Pediatrics, Bioengineering, and Computer Science & Engineering at the University of California San Diego. Humans improve on existing algorithms and lay the groundwork for the future Not only have the gamers improved on the results produced by the existing programs used to analyze DNA sequences, but they are also helping lay the groundwork for improved AI programs that can be used in the future. “We didn’t know whether the players of a popular game like Borderlands 3 would be interested or whether the results would be good enough to improve on what was already known about microbial evolution. But we’ve been amazed by the results,” says Jérôme Waldispühl, an associate professor in McGill’s School of Computer Science and senior author on the paper published today. “In half a day, the Borderlands Science players collected five times more data about microbial DNA sequences than our earlier game, Phylo, had collected over a 10-year period.” The idea for integrating DNA analysis into a commercial video game with mass market appeal came from Attila Szantner, an adjunct professor in McGill’s School of Computer Science and CEO and co-founder of MMOS. “As almost half of the world population is playing with video games, it is of utmost importance that we find new creative ways to extract value from all this time and brainpower that we spend gaming,” says Szantner. “Borderlands Science shows how far we can get by teaming up with the game industry and its communities to tackle the big challenges of our times.” “Gearbox’s developers were eager to engage millions of Borderlands players globally with our creation of an appealing in-game experience to demonstrate how clever minds playing Borderlands are capable of producing tangible, useful, and valuable scientific data at a level not approachable with non-interactive technology and mediums,” said Randy Pitchford, founder and CEO of Gearbox Entertainment Company. “I’m proud that Borderlands Science has become one of the largest and most accomplished citizen science projects of all time, forecasting the opportunity for similar projects in future video games and pushing the boundaries of the positive effect that video games can make on the world.” Relating microbes to disease and lifestyle The tens of trillions of microbes that colonize our bodies play a crucial role in maintaining human health. But microbial communities can change over time in response to factors such as diet, medications, and lifestyle habits. Because of the sheer number of microbes involved, scientists are still only in the early days of being able to identify which microorganisms are affected by, or can affect, which conditions. Which is why the researchers’ project and the results from the gamers are so important. “We expect to be able to use this information to relate specific kinds of microbes to what we eat, to how we age, and to the many diseases ranging from inflammatory bowel disease to Alzheimer’s that we now know microbes to be involved in,” adds Knight, who also directs the Center for Microbiome Innovation at the UC San Diego. “Because evolution is a great guide to function, having a better tree relating our microbes to one another gives us a more precise view of what they are doing within and around us.” Building communities to advance knowledge “Here we have 4.5 million people who contributed to science. In a sense, this result is theirs too and they should feel proud about it,” says Waldispühl. “It shows that we can fight the fear or misconceptions that members of the public may have about science and start building communities who work collectively to advance knowledge.” “Borderlands Science created an incredible opportunity to engage with citizen scientists on a novel and important problem, using data generated by a separate massive citizen science project,” adds Daniel McDonald, the Scientific Director of the Microsetta Initiative. “These results demonstrate the remarkable value of open access data, and the scale of what is possible with inclusive practices in scientific endeavors.” Reference: “Improving microbial phylogeny with citizen science within a mass-market video game” by Roman Sarrazin-Gendron, Parham Ghasemloo Gheidari, Alexander Butyaev, Timothy Keding, Eddie Cai, Jiayue Zheng, Renata Mutalova, Julien Mounthanyvong, Yuxue Zhu, Elena Nazarova, Chrisostomos Drogaris, Kornél Erhart, Borderlands Science Development Team, Borderlands Science players, Amélie Brouillette, Gabriel Richard, Randy Pitchford, Sébastien Caisse, Mathieu Blanchette, Daniel McDonald, Rob Knight, Attila Szantner and Jérôme Waldispühl, 15 April 2024, Nature Biotechnology. DOI: 10.1038/s41587-024-02175-6 The research was funded in part by Genome Canada and Génome Québec. Through introducing plant enzymes that can catalyze key steps along the molecular production line of artepillin C into yeast cells, and by tuning the balance of precursor molecules, the team around Kobe University bioengineer Tomohisa Hasunuma produced artepillin C in fermenters at unprecedented concentrations. Credit: Kobe University Producing herbal medicines on an industrial scale is challenging. However, a team of bioengineers from Kobe University has successfully modified the cellular machinery of a specific yeast species, enabling it to produce a medicinal compound at record concentrations in a fermenter. This breakthrough not only makes large-scale production of this molecule possible but also opens the door to microbial production of other plant-derived compounds. Bioengineered Medicinal Compounds Herbal medicines offer many health benefits, but they are often difficult to produce on a large scale. A good example is artepillin C—a compound with antimicrobial, anti-inflammatory, antioxidant, and anticancer properties—which is currently only available as a bee-derived product. Kobe University bioengineer Tomohisa Hasunuma explains, “To obtain a high-yield and low-cost supply, it is desirable to produce it in bioengineered microorganisms which can be grown in fermenters.” However, this approach presents technical challenges. The yeast Komagataella phaffii is well-suited to produce components for the class of chemicals artepillin C belongs to, can be grown at high cell densities, and does not produce alcohol, which limits cell growth. Credit: Takahiro Bamba Challenges in Microbial Production First, scientists need to identify the enzyme, or molecular machinery, that plants use to produce the target compound. “The plant enzyme that’s key to artepillin C production had only recently been discovered by Kazufumi Yazaki at Kyoto University,” Hasunuma notes. “He asked us whether we can use it to produce the compound in microorganisms due to our experience with microbial production.” The team then worked to introduce the gene responsible for this enzyme into the yeast Komagataella phaffii. Compared to brewer’s yeast, K. phaffii is better suited to produce chemicals in this class, supports higher cell densities, and doesn’t produce alcohol, which can restrict cell growth. Achievements in Artepillin C Production In the journal ACS Synthetic Biology, they now report that their bioengineered yeast produced ten times as much artepillin C as could be achieved before. They accomplished this feat by carefully tuning key steps along the molecular production line of artepillin C. Hasunuma adds: “Another interesting aspect is that artepillin C is not excreted into the growth medium readily and tends to accumulate inside the cell. It was therefore necessary to grow the yeast cells in our fermenters to high densities, which we achieved by removing some of the mutations introduced for technical reasons but that stand in the way of the organism’s dense growth.” The Kobe University bioengineer Tomohisa Hasunuma explains, “Since thousands of compounds with a very similar chemical structure exist naturally, there is the very real possibility that the knowledge gained from the production of artepillin C can be applied to the microbial production of other plant-derived compounds.” Credit: Takahiro Bamba Future Prospects and Broader Implications The Kobe University bioengineer already has ideas how to further improve the production. One approach will be to further raise the efficiency of the final and critical chemical step by modifying the responsible enzyme or by increasing the pool of precursor chemicals. Another approach may be to find a way of transporting artepillin C out of the cell. “If we can modify a transporter, a molecular structure that transports chemicals in and out of cells, such that it exports the product into the medium while keeping the precursors in the cell, we could achieve even higher yields,” Hasunuma says. The implications of this study, however, go beyond the production of this particular compound. Hasunuma explains, “Since thousands of compounds with a very similar chemical structure exist naturally, there is the very real possibility that the knowledge gained from the production of artepillin C can be applied to the microbial production of other plant-derived compounds.” Reference: “De Novo Production of the Bioactive Phenylpropanoid Artepillin C Using Membrane-Bound Prenyltransferase in Komagataella phaffii” by Takahiro Bamba, Ryosuke Munakata, Yuya Ushiro, Ryota Kumokita, Sayaka Tanaka, Yoshimi Hori, Akihiko Kondo, Kazufumi Yazaki and Tomohisa Hasunuma, 12 November 2024, ACS Synthetic Biology. DOI: 10.1021/acssynbio.4c00472 This research was funded by the Japan Society for the Promotion of Science (grant 23H04967), the RIKEN Cluster for Science, Technology and Innovation Hub and the Japan Science and Technology Agency (grant JPMJGX23B4). It was conducted in collaboration with researchers from Kyoto University and the RIKEN Center for Sustainable Resource Science. 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