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KoDō 和牛燒肉適合跨年聚餐嗎?》台中公益路美食特輯|10家真實體驗分享 |
| 知識學習|考試升學 2026/04/20 18:19:55 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人,臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」,從精緻西餐到創意火鍋,從日式丼飯到義式早午餐,每走幾步,就會有完全不同的特色料理餐廳。 這次我特別花了一整個月,實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店,也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準,整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你,找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型,從高質感牛排館到巷弄系早午餐,每一間都有自己獨特的風格。為了讓整體比較更客觀,我依照以下四大面向進行評比,並搭配實際用餐體驗來打分。
整體而言,我希望這份評比不只是「哪家好吃」,而是幫你在不同情境下(約會、家庭聚餐、朋友小聚、商業午餐)都能快速找到合適的選擇。畢竟,美食不只是味覺的滿足,更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段,從火鍋、燒肉到中式料理與早午餐,每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單,橫跨平價、創意、高級各路風格。
一頭牛日式燒肉|炭香濃郁的和牛饗宴,約會聚餐首選
走在公益路上,很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面,搭配昏黃燈光與暖色調的內裝,讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大,但桌距規劃得宜,每桌皆設有獨立排煙設備,烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂,絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網,讓人完全不用分心。整場用餐過程就像一場表演,從視覺、嗅覺到味覺都被滿足。 綜合評分
地址:408臺中市南屯區公益路二段162號電話:04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」,更像是一場五感盛宴。從進門那一刻到最後一道甜點,都能感受到他們對細節的用心。 TANG Zhan 湯棧|文青系火鍋代表,麻香湯底與視覺美感並重
在公益路這條美食戰線上,TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址:408臺中市南屯區公益路二段248號電話:04-22580617 官網:https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度,又結合現代風格與細節服務,讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店,湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店|明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂,那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主,陽光灑進室內,讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧,建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味,選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口,米芯保留微Q口感;而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香,口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹,無論是一個人帶電腦工作、或朋友聚餐,都能找到舒服角落。餐點上桌速度穩定,服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」,很適合想遠離忙碌日常的人。 綜合評分
地址:40861臺中市南屯區公益路二段18號電話:04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇,而是以「剛剛好」的份量與風味,陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店,NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物|平價卻用心的湯頭系火鍋,家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上,加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐,但靠著實在的湯頭與親切的服務,默默吸引許多回頭客。每到用餐時間,總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底,湯頭清澈卻不單薄,越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向,桌距寬敞、座位舒適,帶小孩來也不覺擁擠。店員態度親切,補湯、收盤都很勤快,給人一種「被照顧著」的安心感。 綜合評分
地址:403臺中市西區公益路288號電話:0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤,而是用最簡單的湯頭與新鮮食材,傳遞出家常卻不平凡的溫度。 印月餐廳|中式料理的藝術演繹,宴客與家庭聚會首選
說到臺中公益路的中式料理代表,印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名,把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設,每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏,都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法,讓人感受到「被款待」的尊榮感。 綜合評分
地址:408臺中市南屯區公益路二段818號電話:0422511155 小結語印月餐廳是一間「不只吃飯,更像品味生活」的地方。 KoDō 和牛燒肉|極致職人精神,專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗,一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉,以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址:403臺中市西區公益路260號電話:0423220312 官網:https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳,而是一場體驗。 永心鳳茶|在茶香裡用餐的優雅時光,臺味早午餐的新詮釋
走進 永心鳳茶公益店,彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤,無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」,都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和,對茶品介紹詳盡。上餐節奏剛好,不急不徐。 綜合評分
地址:40360臺中市西區公益路68號三樓(勤美誠品)電話:0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓|老饕級江浙功夫菜,穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主,兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址:408臺中市南屯區公益路二段95號電話:0423202322 官網:https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司|低調奢華的無菜單日料,職人手藝詮釋旬味極致
在熱鬧的公益路上,一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase(無菜單料理) 形式,每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘,節奏緩慢但沉穩。 綜合評分
地址:408臺中市南屯區公益路二段25號電話:0423206368 官網:https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂|人氣爆棚的和牛燒肉聖地,肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳,茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐,價格約落在 $700–$1000 間,份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快,整場用餐流程流暢無壓力。 綜合評分
地址:403臺中市西區公益路268號電話:0423281167 官網:https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質+輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後,臺中公益路不只是一條美食街,而是一段生活風景線。 有的餐廳講究細膩與儀式感,像 一頭牛日式燒肉 與 一笈壽司,讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心,像 加分昆布鍋物、永心鳳茶,讓人明白吃飯不只是為了飽足,而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店,則用穩定的品質與熱絡的氛圍,成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店,構成了公益路最動人的縮影 有華麗的,也有溫柔的;有傳統的,也有創新的。 每一家都在自己的風格裡發光,讓人吃到的不只是料理,而是一種生活的溫度與節奏。 對我而言,這不僅是一場美食旅程,更是一趟關於「臺中味道」的回憶之旅。 FAQ:關於臺中公益路美食常見問題Q1:公益路哪一區的餐廳最集中? Q2:需要提前訂位嗎? 最後的話若要用一句話形容這趟美食之旅,我會說: 一笈壽司價格合理嗎? 如果你也和我一樣喜歡用味蕾探索一座城市,那就把這篇公益路美食攻略收藏起來吧。永心鳳茶尾牙拍照效果好嗎? 無論是約會、慶生、家庭聚餐,或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。一笈壽司小資族值得嗎? 下一餐,不妨從這10家開始。一頭牛日式燒肉節慶時段會不會太難訂位? 打開手機、約上朋友,讓公益路成為你生活裡最容易抵達的小確幸。一頭牛日式燒肉小孩適合去嗎? 如果你有私心愛店,也歡迎留言分享,TANG Zhan 湯棧需要訂位嗎? 你的推薦,可能讓我下一趟美食旅程變得更精彩。永心鳳茶年節期間價格會變嗎? A rosy wolf snail marked and equipped with a Michigan Micro Mote computer system in the Fautaua-Iti Valley site in Tahiti. Credit: Inhee Lee Study yields new insights into the survival of a native snail important to Tahitian culture and ecology and to biologists studying evolution. More than 50 species of tree snail in the South Pacific Society Islands were wiped out following the introduction of an alien predatory snail in the 1970s, but the white-shelled Partula hyalina survived. Now, thanks to a collaboration between University of Michigan biologists and engineers with the world’s smallest computer, scientists understand why: P. hyalina can tolerate more sunlight than its predator, so it was able to persist in sunlit forest edge habitats. “We were able to get data that nobody had been able to obtain,” said David Blaauw, the Kensall D. Wise Collegiate Professor of Electrical Engineering and Computer Science. “And that’s because we had a tiny computing system that was small enough to stick on a snail.” The Michigan Micro Mote (M3), considered the world’s smallest complete computer, was announced in 2014 by a team Blaauw co-led. This was its first field application. A Partula hyalina snail resting on a wild red ginger leaf next to a Michigan Micro Mote computer system in a forest edge habitat in Tahiti. Credit: Inhee Lee “The sensing computers are helping us understand how to protect endemic species on islands,” said Cindy Bick, who received a Ph.D. in ecology and evolutionary biology from U-M in 2018. “If we are able to map and protect these habitats through appropriate conservation measures, we can figure out ways to ensure the survival of the species.” P. hyalina is important culturally for Polynesians because of its unique color, making it attractive for use in shell leis and jewelry. Tree snails also play a vital role in island forest ecosystems, as the dominant group of native grazers. How Society Island snails were wiped out The giant African land snail was introduced to the Society Islands, including Tahiti, to cultivate as a food source, but it became a major pest. To control its population, agricultural scientists introduced the rosy wolf snail in 1974. But unfortunately, most of the 61 known species of native Society Islands tree snails were easy prey for the rosy wolf. P. hyalina is one of only five survivors in the wild. Called the “Darwin finches of the snail world” for their island-bound diversity, the loss of so many Partula species is a blow to biologists studying evolution. “The endemic tree snails had never encountered a predator like the alien rosy wolf snail before it’s deliberate introduction. It can climb trees and very quickly drove most of the valley populations to local extinction,” said Diarmaid Ó Foighil, professor of ecology and evolutionary biology and curator of the U-M Museum of Zoology. Inhee Lee, an assistant professor at the University of Pittsburgh and alum of Michigan Engineering, attaches a Michigan Micro Mote computer system to a leaf harboring a Partula hyalina snail. Credit: Cindy Bick In 2015, Ó Foighil and Bick hypothesized that P. hyalina‘s distinctive white shell might give it an important advantage in forest edge habitats, by reflecting rather than absorbing light radiation levels that would be deadly to its darker-shelled predator. To test their idea, they needed to be able to track the light exposure levels P. hyalina and rosy wolf snails experienced in a typical day. Field work in Tahiti shows P. hyalina can take 10x more light Bick and Ó Foighil wanted to attach light sensors to the snails, but a system made using commercially available chips would have been too big. Bick found news of a smart sensor system that was just 2x5x2 mm, and the developers were at her own institution. But could it be altered to sense light? “It was important to understand what the biologists were thinking and what they needed,” said Inhee Lee, an assistant professor of electrical and computer engineering at the University of Pittsburgh who received a Ph.D. from U-M electrical and computer engineering in 2014. Lee adapted the M3 for the study. The Fautaua-lti Valley site in Tahiti where this research was conducted includes an open trail through the rainforest. The predator rosy wolf snail was located and tracked on both sides of the trail. Credit: Cindy Bick The first step was to figure out how to measure the light intensity of the snails’ habitats. At the time, the team had just added an energy harvester to the M3 system to recharge the battery using tiny solar cells. Lee realized he could measure the light level continuously by measuring the speed at which the battery was charging. After testing enabled by local Michigan snails, 50 M3s made it to Tahiti in 2017. Bick and Lee joined forces with Trevor Coote, a well-known conservation field biologist and specialist on the French Polynesian snails. The team glued the sensors directly to the rosy wolf snails, but P. hyalina is a protected species and required an indirect approach. They are nocturnal, typically sleeping during the day while attached underneath leaves. Using magnets, the team placed M3s both on the tops and undersides of leaves harboring the resting P. hyalina. At the end of each day, Lee wirelessly downloaded the data from each of the M3s. Cindy Bick checks the flora in the Tipaerui-lli Valley site in Tahiti, an edge forest habitat where the Partula hyalina snails survive. Credit: Trevor Coote During the noon hour, the P. hyalina habitat received on average 10 times more sunlight than the rosy wolf snails. The researchers suspect that the rosy wolf doesn’t venture far enough into the forest edge to catch P. hyalina, even under cover of darkness, because they wouldn’t be able to escape to shade before the sun became too hot. “The M3 really opens up the window of what we can do with invertebrate behavioral ecology and we’re just at the foothills of those possibilities,” Ó Foighil said. Reference: “Millimeter-sized smart sensors reveal that a solar refuge protects tree snail Partula hyalina from extirpation” by Cindy S. Bick, Inhee Lee, Trevor Coote, Amanda E. Haponski, David Blaauw and Diarmaid Ó Foighil, 15 June 2021, Communications Biology. DOI: 10.1038/s42003-021-02124-y This project has already facilitated a subsequent collaboration between engineering and ecology and evolutionary biology tracking monarch butterflies. The article in the journal Communications Biology is titled, “Millimeter-sized smart sensors reveal that a solar refuge protects tree snail Partula hyalina from extirpation.” The project was supported by U-M’s MCubed program, created to stimulate and support innovative research among interdisciplinary teams. Additional funding was provided by the Department of Ecology and Evolutionary Biology and by National Science Foundation and Arm Ltd. funding to the Blaauw lab. The whale shark is brought shallower to the surface and one orca is biting near the pelvic area. Credit: Kelsey Williamson Researchers have observed orcas in the Gulf of California using collaborative techniques to hunt whale sharks by targeting their less protected areas. This specialized behavior suggests an evolutionary adaptation in their hunting strategies. As the apex predators of the ocean, killer whales feed on a wide variety of prey, including marine mammals, turtles, and fish. In the Gulf of California, a pod may have acquired new skills that help them hunt whale sharks – the world’s largest fish. Whale sharks, which can grow up to 18 meters long, feed at aggregation sites in the Gulf of California, occasionally while still young and relatively small. At this vulnerable life stage, they are more susceptible to predation, and there is anecdotal evidence that orcas may be hunting them. Now, researchers in Mexico have reported four separate hunting events. Killer whales surface to breath before taking whale shark down and delivering a rapid, final blow. Credit: Kelsey Williamson “We show how orcas displayed a collaboratively hunting technique on whale sharks, characterized by focusing on attacking the pelvic area causing the whale shark to bleed out and allow orcas access to the lipid-rich liver,” said Erick Higuera Rivas, a marine biologist at Conexiones Terramar and senior author of the Frontiers in Marine Science article. “One individual was engaged in three of the four events, alongside with other members who might belong to a pod specialized on hunting on sharks.” The killer whale “Moctezuma” approaching the whale shark that was still moving and upside down at the surface. Credit: Eduardo Miranda. Specialized Hunters The predation events occurred between 2018 and 2024 in the southern Gulf of California and were captured in images and videos taken by members of the public and scientists. Individual orcas were identified by analyzing photographs of dorsal fins and distinctive features like scars. In three of four hunting events a male orca called Moctezuma was present. A female orca previously observed in the presence of Moctezuma participated in one event also, suggesting they could be related or members of the same pod. Killer whale hits the shark with the side of its head. Credit: Kelsey Williamson “When hunting, all pod members work together, hitting the whale shark to turn it upside down. In that position the sharks enter a state of tonic immobility and can no longer move voluntarily or escape by going deeper,” Higuera Rivas explained. “By keeping it under control, the orcas then have greater ease and speed in approaching the pelvic area of the shark and are able to extract organs of nutritional importance for them.” Orcas may be targeting whale sharks’ ventral side because their bodies are thought to be the least protected. For example, there is less muscle and cartilage in this area, allowing easier access to the aorta. Although whale sharks’ livers are an important part of orcas’ diets, there was no photographic observation of the orcas consuming the organ. Hunting this way could imply that some orcas in the Gulf of California have acquired special skills that help them prey on whale sharks. In other regions, orcas may have learned to do the same thing, but evidence is limited, the researchers said. The adult male killer whale Moctezuma and five other killer whales (two adult females, two juveniles, one calf), in the vicinity of the juvenile whale shark, measuring around 6 meters. The whale shark was observed to be weakly swimming at the surface. Credit: Guillermo Aceves Salazar Understanding How Orcas Hunt Collecting the data the scientists needed wasn’t easy. Attacks cannot be predicted, access to images and videos can be limited, and image quality is oftentimes too low to reliably identify animals. Despite this, the researchers said their findings could have several implications. The fact that there is a whale shark hunting pod in the Gulf of California increases the need for managing marine adventurers and tourists, the researchers cautioned. “There must be a specific regulatory norm that guarantees that any type of non-extractive use activity is carried out in a respectful and sustainable manner,” Higuera Rivas said. Several of the killer whales were observed with whale shark carrion in their mouths during an attack on May 26, 2024. Credit: Kelsey Williamson Further, if the thesis that Moctezuma and his pod have acquired ecological and behavioral information for hunting whale sharks in the Gulf of California holds, the pod could be vulnerable to a possible disappearance of this specific prey due to climate change within the region. Ultimately, new information related to these orcas helps researchers gain a greater understanding of their adaptations unique to their needs and geographical location. “It is very impressive how orcas work together strategically and intelligently to access only a very specific area of the prey. It highlights what great predators they are,” concluded Higuera Rivas. Moctezuma, the adult male of the pod is identified as one of the killer whales involved in the attack. Credit: James Moskito Reference: “Killer whales (Orcinus orca) hunt, kill and consume the largest fish on Earth, the whale shark (Rhincodon typus)” by Francesca Pancaldi, Kathryn A. Ayres, Austin J. Gallagher, James Moskito, Kelsey C. Williamson and Jesús Erick Higuera Rivas, 23 September 2024, Frontiers in Marine Science. DOI: 10.3389/fmars.2024.1448254 A simplified synthetic equivalent of a cell would be like a blueprint for life. Credit: EVOLF project Scientists are designing simplified biological systems, aiming to construct synthetic cells and better understand life’s mechanisms. One of the most fundamental questions in science is how lifeless molecules can come together to form a living cell. Bert Poolman, Professor of Biochemistry at the University of Groningen, has been working to solve this problem for two decades. He aims to understand life by trying to reconstruct it; he is building simplified artificial versions of biological systems that can be used as components for a synthetic cell. This is Bert Poolman, Professor of Biochemistry at the University of Groningen. Credit: University of Groningen His work was detailed in two new papers published in Nature Nanotechnology and Nature Communications. In the first paper, he describes a system for energy conversion and cross-feeding of products of this reaction between synthetic cells, while he describes a system for concentrating and converting nutrients in cells in the second paper. Synthetic Cells and Energy Conversion Six Dutch research institutes are collaborating in the consortium BaSyc (Building a Synthetic Cell) to build the elements needed for a synthetic cell. Poolman’s group has been working on energy conversion. The real-life equivalents he aims to replicate are mitochondria, the “energy factories” of the cell. These use the molecule ADP to produce ATP, which is the standard “fuel” that cells require to function. When ATP is converted back into ADP, the energy is released and used to drive other processes. This image shows the production of ATP in a mitochondrion, requiring > 100 proteins, and the synthetic vesicles producing ATP, with only 5 proteins. CreditUniversity of Groningen, Poolman lab Artificial Energy Factories “Instead of the hundreds of components of mitochondria, our system for energy conversion uses just five,” says Poolman. “We set out to simplify it as much as possible.” This may sound odd, as evolution has done a great job of producing functional systems. “However, evolution is a one-way street, it builds on existing components and this often makes the outcome very complex,” explains Poolman. An artificial replica, on the other hand, can be designed with a specific outcome in mind. The five components were placed inside vesicles, tiny cell-like sacs, that can absorb ADP as well as the amino acid arginine from the surrounding fluid. The arginine is “burned” (deaminated) and thus provides the energy to produce ATP, which is secreted from the vesicle. “Of course, the simplification comes at a price: we can only use arginine as the energy source, while cells use all kinds of different molecules, such as amino acids, fats, and sugars.” Biological systems serve as examples for the simplified artificial systems used to create synthetic cells. Credit: Beeld Willy Arisky via Pexels Next, the Poolman group designed a second vesicle that can absorb the secreted ATP and use it to drive an energy-consuming reaction. The energy is provided by turning ATP back into ADP, which is then secreted and can be absorbed by the first vesicle, closing the loop. Such a cycle of ATP production and use is the foundation of metabolism in every living cell and drives the “machinery” for energy-consuming reactions such as growth, cell division, protein synthesis, DNA replication, and more. An Artificial Pumping System The second module that Poolman created was a bit different: a vesicle in which a chemical process causes the interior to build up a negative charge and, in doing so, form an electrical potential, similar to that of an electronic circuit. The electrical potential is used to couple charge movement to the accumulation of nutrients inside the vesicle, which is carried out by transporters. These proteins in the membrane of the vesicle work a bit like a water wheel: positively charged protons “flow” through it from outside the vesicle to the negatively charged interior. This flow drives the transporter, which in this case imports a sugar molecule, lactose. Again, this is a very common process in living cells, requiring many components that Poolman and his team mimicked with just two components. The cells of our muscles are also powered by ATP. Credit: Victor Freitas When he submitted a paper describing this system, a reviewer asked if he couldn’t do something with the lactose that is being transported, as cells use nutrients like this to produce useful building blocks. Poolman took up the challenge and added three more enzymes to the system, which oxidized the sugar and enabled the production of the coenzyme NADH. “This helper molecule plays an essential role in the proper functioning of all cells,” explains Poolman. “And by adding NADH production, we have shown that it is feasible to expand the system.” Towards a Fully Functional Synthetic Cell Having a simplified synthetic equivalent of two key features of life is fascinating, but many more steps need to be integrated to form an autonomously growing and dividing synthetic cell. “The next step we want to take is adding our metabolic energy producing systems to a synthetic cell division system created by colleagues,” says Poolman. The BaSyc program is entering its final years; funding for a new program has recently been secured. A large consortium of Dutch groups, in which Poolman is one of the leading scientists, received 40 million euros to create life from non-living modules. This EVOLF project is set to run for another ten years and aims to find out how many more lifeless modules can come together and create living cells. “Ultimately, this would give us a blueprint for life, something that is currently lacking in biology,” concludes Poolman. “This may eventually have all kinds of applications, but will also help us to better understand what life is.” References: “Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation” by Miyer F. Patiño-Ruiz, Zaid Ramdhan Anshari, Bauke Gaastra, Dirk J. Slotboom and Bert Poolman, 12 September 2024, Nature Communications. DOI: 10.1038/s41467-024-52085-z “Synthetic syntrophy for adenine nucleotide cross-feeding between metabolically active nanoreactors” by Laura Heinen, Marco van den Noort, Martin S. King, Edmund R. S. Kunji and Bert Poolman, 21 October 2024, Nature Nanotechnology. DOI: 10.1038/s41565-024-01811-1 RRG455KLJIEVEWWF |
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