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文章數：103 |
 TANG Zhan 湯棧需要訂位嗎？》公益路餐廳完整攻略｜10大人氣店家解析 |
| 時事評論｜政治 2026/04/22 12:46:46 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人，臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」，從精緻西餐到創意火鍋，從日式丼飯到義式早午餐，每走幾步，就會有完全不同的特色料理餐廳。 這次我特別花了一整個月，實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店，也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準，整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你，找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型，從高質感牛排館到巷弄系早午餐，每一間都有自己獨特的風格。為了讓整體比較更客觀，我依照以下四大面向進行評比，並搭配實際用餐體驗來打分。
整體而言，我希望這份評比不只是「哪家好吃」，而是幫你在不同情境下（約會、家庭聚餐、朋友小聚、商業午餐）都能快速找到合適的選擇。畢竟，美食不只是味覺的滿足，更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段，從火鍋、燒肉到中式料理與早午餐，每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單，橫跨平價、創意、高級各路風格。
一頭牛日式燒肉｜炭香濃郁的和牛饗宴，約會聚餐首選
走在公益路上，很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面，搭配昏黃燈光與暖色調的內裝，讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大，但桌距規劃得宜，每桌皆設有獨立排煙設備，烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂，絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網，讓人完全不用分心。整場用餐過程就像一場表演，從視覺、嗅覺到味覺都被滿足。 綜合評分
地址：408臺中市南屯區公益路二段162號電話：04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」，更像是一場五感盛宴。從進門那一刻到最後一道甜點，都能感受到他們對細節的用心。 TANG Zhan 湯棧｜文青系火鍋代表，麻香湯底與視覺美感並重
在公益路這條美食戰線上，TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址：408臺中市南屯區公益路二段248號電話：04-22580617 官網：https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度，又結合現代風格與細節服務，讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店，湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店｜明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂，那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主，陽光灑進室內，讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧，建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味，選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口，米芯保留微Q口感；而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香，口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹，無論是一個人帶電腦工作、或朋友聚餐，都能找到舒服角落。餐點上桌速度穩定，服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」，很適合想遠離忙碌日常的人。 綜合評分
地址：40861臺中市南屯區公益路二段18號電話：04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇，而是以「剛剛好」的份量與風味，陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店，NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物｜平價卻用心的湯頭系火鍋，家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上，加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐，但靠著實在的湯頭與親切的服務，默默吸引許多回頭客。每到用餐時間，總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底，湯頭清澈卻不單薄，越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向，桌距寬敞、座位舒適，帶小孩來也不覺擁擠。店員態度親切，補湯、收盤都很勤快，給人一種「被照顧著」的安心感。 綜合評分
地址：403臺中市西區公益路288號電話：0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤，而是用最簡單的湯頭與新鮮食材，傳遞出家常卻不平凡的溫度。 印月餐廳｜中式料理的藝術演繹，宴客與家庭聚會首選
說到臺中公益路的中式料理代表，印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名，把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設，每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏，都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法，讓人感受到「被款待」的尊榮感。 綜合評分
地址：408臺中市南屯區公益路二段818號電話：0422511155 小結語印月餐廳是一間「不只吃飯，更像品味生活」的地方。 KoDō 和牛燒肉｜極致職人精神，專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗，一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉，以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址：403臺中市西區公益路260號電話：0423220312 官網：https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳，而是一場體驗。 永心鳳茶｜在茶香裡用餐的優雅時光，臺味早午餐的新詮釋
走進 永心鳳茶公益店，彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤，無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」，都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和，對茶品介紹詳盡。上餐節奏剛好，不急不徐。 綜合評分
地址：40360臺中市西區公益路68號三樓(勤美誠品)電話：0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓｜老饕級江浙功夫菜，穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主，兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址：408臺中市南屯區公益路二段95號電話：0423202322 官網：https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司｜低調奢華的無菜單日料，職人手藝詮釋旬味極致
在熱鬧的公益路上，一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase（無菜單料理） 形式，每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘，節奏緩慢但沉穩。 綜合評分
地址：408臺中市南屯區公益路二段25號電話：0423206368 官網：https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂｜人氣爆棚的和牛燒肉聖地，肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳，茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐，價格約落在 $700–$1000 間，份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快，整場用餐流程流暢無壓力。 綜合評分
地址：403臺中市西區公益路268號電話：0423281167 官網：https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質＋輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後，臺中公益路不只是一條美食街，而是一段生活風景線。 有的餐廳講究細膩與儀式感，像 一頭牛日式燒肉 與 一笈壽司，讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心，像 加分昆布鍋物、永心鳳茶，讓人明白吃飯不只是為了飽足，而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店，則用穩定的品質與熱絡的氛圍，成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店，構成了公益路最動人的縮影 有華麗的，也有溫柔的；有傳統的，也有創新的。 每一家都在自己的風格裡發光，讓人吃到的不只是料理，而是一種生活的溫度與節奏。 對我而言，這不僅是一場美食旅程，更是一趟關於「臺中味道」的回憶之旅。 FAQ：關於臺中公益路美食常見問題Q1：公益路哪一區的餐廳最集中？ Q2：需要提前訂位嗎？ 最後的話若要用一句話形容這趟美食之旅，我會說： 一笈壽司清淡口味適合嗎？ 如果你也和我一樣喜歡用味蕾探索一座城市，那就把這篇公益路美食攻略收藏起來吧。三希樓尾牙聚餐表現如何？ 無論是約會、慶生、家庭聚餐，或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。一笈壽司包廂適合尾牙嗎？ 下一餐，不妨從這10家開始。印月餐廳過年期間會開門嗎？ 打開手機、約上朋友，讓公益路成為你生活裡最容易抵達的小確幸。NINI 尼尼臺中店好吃嗎？ 如果你有私心愛店，也歡迎留言分享，KoDō 和牛燒肉適合請客嗎？ 你的推薦，可能讓我下一趟美食旅程變得更精彩。茶六燒肉堂情侶來合適嗎？ A new study utilizing advanced Neuropixels probes, provides insights into how the brain’s neurons enable the formulation and verbal expression of thoughts, revealing the pre-verbal planning of speech sounds. This breakthrough research, offering potential for developing speech prosthetics and enhancing treatments for language disorders, underscores the complexity and efficiency of the brain’s language production capabilities. Results may be utilized to create innovative therapies for speech and language impairments. A recent study conducted by Massachusetts General Hospital (MGH) researchers has utilized sophisticated brain recording methods to reveal the collaborative function of neurons in the human brain, enabling individuals to formulate their thoughts into words and subsequently articulate them verbally. Together, these findings provide a detailed map of how speech sounds such as consonants and vowels are represented in the brain well before they are even spoken and how they are strung together during language production. The work, which is published in Nature, reveals insights into the brain’s neurons that enable language production, and which could lead to improvements in the understanding and treatment of speech and language disorders. “Although speaking usually seems easy, our brains perform many complex cognitive steps in the production of natural speech—including coming up with the words we want to say, planning the articulatory movements, and producing our intended vocalizations,” says senior author Ziv Williams, MD, an associate professor in Neurosurgery at MGH and Harvard Medical School. “Our brains perform these feats surprisingly fast—about three words per second in natural speech—with remarkably few errors. Yet how we precisely achieve this feat has remained a mystery.” Technological Breakthroughs in Neuronal Recording When they used a cutting-edge technology called Neuropixels probes to record the activities of single neurons in the prefrontal cortex, a frontal region of the human brain, Williams and his colleagues identified cells that are involved in language production and that may underlie the ability to speak. They also found that there are separate groups of neurons in the brain dedicated to speaking and listening. “The use of Neuropixels probes in humans was first pioneered at MGH. These probes are remarkable—they are smaller than the width of a human hair, yet they also have hundreds of channels that are capable of simultaneously recording the activity of dozens or even hundreds of individual neurons,” says Williams who had worked to develop these recording techniques with Sydney Cash, MD, PhD, a professor in Neurology at MGH and Harvard Medical School, who also helped lead the study. “Use of these probes can therefore offer unprecedented new insights into how neurons in humans collectively act and how they work together to produce complex human behaviors such as language.” Decoding Speech Elements The study showed how neurons in the brain represent some of the most basic elements involved in constructing spoken words—from simple speech sounds called phonemes to their assembly into more complex strings such as syllables. For example, the consonant “da”, which is produced by touching the tongue to the hard palate behind the teeth, is needed to produce the word dog. By recording individual neurons, the researchers found that certain neurons become active before this phoneme is spoken out loud. Other neurons reflected more complex aspects of word construction such as the specific assembly of phonemes into syllables. With their technology, the investigators showed that it’s possible to reliably determine the speech sounds that individuals will say before they articulate them. In other words, scientists can predict what combination of consonants and vowels will be produced before the words are actually spoken. This capability could be leveraged to build artificial prosthetics or brain-machine interfaces capable of producing synthetic speech, which could benefit a range of patients. “Disruptions in the speech and language networks are observed in a wide variety of neurological disorders—including stroke, traumatic brain injury, tumors, neurodegenerative disorders, neurodevelopmental disorders, and more,” says Arjun Khanna who is a co-author on the study. “Our hope is that a better understanding of the basic neural circuitry that enables speech and language will pave the way for the development of treatments for these disorders.” The researchers hope to expand on their work by studying more complex language processes that will allow them to investigate questions related to how people choose the words that they intend to say and how the brain assembles words into sentences that convey an individual’s thoughts and feelings to others. Reference: “Single-neuronal elements of speech production in humans” by Arjun R. Khanna, William Muñoz, Young Joon Kim, Yoav Kfir, Angelique C. Paulk, Mohsen Jamali, Jing Cai, Martina L. Mustroph, Irene Caprara, Richard Hardstone, Mackenna Mejdell, Domokos Meszéna, Abigail Zuckerman, Jeffrey Schweitzer, Sydney Cash and Ziv M. Williams, 31 January 2024, Nature. DOI: 10.1038/s41586-023-06982-w Additional authors include William Muñoz, Young Joon Kim, Yoav Kfir, Angelique C. Paulk, Mohsen Jamali, Jing Cai, Martina L Mustroph, Irene Caprara, Richard Hardstone, Mackenna Mejdell, Domokos Meszena, Abigail Zuckerman, and Jeffrey Schweitzer. This work was supported by the National Institutes of Health. The research team also discovered that protective phenotypes, like the hard shells of most turtle species, might delay the aging process and, in some circumstances, even stop biological aging. The largest study of its kind reveals that wild turtles age slowly, live long lives, and uncovers several species that practically do not age. Jonathan the Seychelles giant tortoise, who is 190 years old, made headlines recently for being the “oldest living land animal in the world.” Although there is anecdotal evidence that certain species of turtles and other ectotherms, or “cold-blooded” creatures, live a long life, this evidence is spotty and mostly focuses on animals kept in zoos or a small number of individuals surviving in the wild. The largest study on aging and lifespan to date, conducted by an international team of 114 scientists and directed by Penn State and Northeastern Illinois University, has recently been published. It contains data gathered in the wild from 107 populations of 77 different species of reptiles and amphibians. A photo of a painted turtle (Chrysemys picta), a widespread North American species of freshwater turtle. Credit: Beth A. Reinke, Northeastern Illinois University The researchers discovered several things, including for the first time, that salamanders, crocodilians, and turtles had extremely slow aging rates and prolonged lifespans for their sizes. They recently published their results in the journal Science. The research team also discovered that protective phenotypes, such as the hard shells of the majority of turtle species, lead to slower aging and, in certain circumstances, even to “negligible aging,” or the absence of biological aging. “Anecdotal evidence exists that some reptiles and amphibians age slowly and have long lifespans, but until now no one has actually studied this on a large scale across numerous species in the wild,” said David Miller, senior author and associate professor of wildlife population ecology, Penn State. “If we can understand what allows some animals to age more slowly, we can better understand aging in humans, and we can also inform conservation strategies for reptiles and amphibians, many of which are threatened or endangered.” Investigating Aging Through Mark-Recapture Data In their study, the researchers used mark-recapture data, in which animals are taken, tagged, released back into the wild, and then watched, in conjunction with comparative phylogenetic approaches, which allow for investigation of organisms’ evolution. Their purpose was to compare ectotherm aging and lifespan in the wild to endotherms (warm-blooded animals) and investigate earlier assumptions about aging, such as manner of body temperature control and the presence or absence of protective physical features. The face of a tuatara (Sphenodon punctatus). Credit: Sarah Lamar Miller explained that the ‘thermoregulatory mode hypothesis’ suggests that ectotherms — because they require external temperatures to regulate their body temperatures and, therefore, often have lower metabolisms — age more slowly than endotherms, which internally generate their own heat and have higher metabolisms. “People tend to think, for example, that mice age quickly because they have high metabolisms, whereas turtles age slowly because they have low metabolisms,” said Miller. The team’s findings, however, reveal that ectotherms’ aging rates and lifespans range both well above and below the known aging rates for similar-sized endotherms, suggesting that the way an animal regulates its temperature — cold-blooded versus warm-blooded — is not necessarily indicative of its aging rate or lifespan. “We didn’t find support for the idea that a lower metabolic rate means ectotherms are aging slower,” said Miller. “That relationship was only true for turtles, which suggests that turtles are unique among ectotherms.” Protective Phenotypes and Slow Aging The protective phenotypes hypothesis suggests that animals with physical or chemical traits that confer protection — such as armor, spines, shells, or venom — have slower aging and greater longevity. The team documented that these protective traits do, indeed, enable animals to age more slowly and, in the case of physical protection, live much longer for their size than those without protective phenotypes. “It could be that their altered morphology with hard shells provides protection and has contributed to the evolution of their life histories, including negligible aging – or lack of demographic aging – and exceptional longevity,” said Anne Bronikowski, co-senior author and professor of integrative biology, Michigan State. Beth Reinke, first author and assistant professor of biology, at Northeastern Illinois University, further explained, “These various protective mechanisms can reduce animals’ mortality rates because they’re not getting eaten by other animals. Thus, they’re more likely to live longer, and that exerts pressure to age more slowly. We found the biggest support for the protective phenotype hypothesis in turtles. Again, this demonstrates that turtles, as a group, are unique.” Negligible Aging Observed Across Multiple Ectotherm Groups Interestingly, the team observed negligible aging in at least one species in each of the ectotherm groups, including frogs and toads, crocodilians, and turtles. An Iberian tree frog (Hyla molleri). Credit: Iñigo Martínez-Solano “It sounds dramatic to say that they don’t age at all, but basically their likelihood of dying does not change with age once they’re past reproduction,” said Reinke. Miller added, “Negligible aging means that if an animal’s chance of dying in a year is 1% at age 10, if it is alive at 100 years, its chance of dying is still 1%. By contrast, in adult females in the U.S., the risk of dying in a year is about 1 in 2,500 at age 10 and 1 in 24 at age 80. When a species exhibits negligible senescence (deterioration), aging just doesn’t happen.” Reinke noted that the team’s novel study was only possible because of the contributions of a large number of collaborators from across the world studying a wide variety of species. “Being able to bring these authors together who have all done years and years of work studying their individual species is what made it possible for us to get these more reliable estimates of aging rate and longevity that are based on population data instead of just individual animals,” she said. Bronikowski added, “Understanding the comparative landscape of aging across animals can reveal flexible traits that may prove worthy targets for biomedical study related to human aging.” Reference: “Diverse aging rates in ectothermic tetrapods provide insights for the evolution of aging and longevity” by Beth A. Reinke, Hugo Cayuela, Fredric J. Janzen, Jean-François Lemaître, Jean-Michel Gaillard, A. Michelle Lawing, John B. Iverson, Ditte G. Christiansen, Iñigo Martínez-Solano, Gregorio Sánchez-Montes, Jorge Gutiérrez-Rodríguez, Francis L. Rose, Nicola Nelson, Susan Keall, Alain J. Crivelli, Theodoros Nazirides, Annegret Grimm-Seyfarth, Klaus Henle, Emiliano Mori, Gaëtan Guiller, Rebecca Homan, Anthony Olivier, Erin Muths, Blake R. Hossack, Xavier Bonnet, David S. Pilliod, Marieke Lettink, Tony Whitaker, Benedikt R. Schmidt, Michael G. Gardner, Marc Cheylan, Françoise Poitevin, Ana Golubovic, Ljiljana Tomovic, Dragan Arsovski, Richard A. Griffiths, Jan W. Arntzen, Jean-Pierre Baron, Jean-François Le Galliard, Thomas Tully, Luca Luiselli, Massimo Capula, Lorenzo Rugiero, Rebecca McCaffery, Lisa A. Eby, Venetia Briggs-Gonzalez, Frank Mazzotti, David Pearson, Brad A. Lambert, David M. Green, Nathalie Jreidini, Claudio Angelini, Graham Pyke, Jean-Marc Thirion, Pierre Joly, Jean-Paul Léna, Anton D. Tucker, Col Limpus, Pauline Priol, Aurélien Besnard, Pauline Bernard, Kristin Stanford, Richard King, Justin Garwood, Jaime Bosch, Franco L. Souza, Jaime Bertoluci, Shirley Famelli, Kurt Grossenbacher, Omar Lenzi, Kathleen Matthews, Sylvain Boitaud, Deanna H. Olson, Tim S. Jessop, Graeme R. Gillespie, Jean Clobert, Murielle Richard, Andrés Valenzuela-Sánchez, Gary M. Fellers, Patrick M. Kleeman, Brian J. Halstead, Evan H. Campbell Grant, Phillip G. Byrne, Thierry Frétey, Bernard Le Garff, Pauline Levionnois, John C. Maerz, Julian Pichenot, Kurtulus Olgun, Nazan Üzüm, Aziz Avci, Claude Miaud, Johan Elmberg, Gregory P. Brown, Richard Shine, Nathan F. Bendik, Lisa O’Donnell, Courtney L. Davis, Michael J. Lannoo, Rochelle M. Stiles, Robert M. Cox, Aaron M. Reedy, Daniel A. Warner, Eric Bonnaire, Kristine Grayson, Roberto Ramos-Targarona, Eyup Baskale, David Muñoz, John Measey, F. Andre de Villiers, Will Selman, Victor Ronget, Anne M. Bronikowski and David A. W. Miller, 23 June 2022, Science. DOI: 10.1126/science.abm0151 The study was funded by the National Institutes of Health. Ph.D. candidate Vasin Dumrongprechachan captures protein expression in the mouse brain for mass spectrometry analysis, visualized by fluorescence microscopy. Images shows a sagittal cross section of the mouse striatum. Blue is the outline of the brain. Green and magenta show selectively tagged proteins for mass spectrometry analysis. Credit: Northwestern University Tool could help researchers better understand brain diseases and potential treatments. For the first time, researchers have developed a successful approach for identifying proteins inside different types of neurons in the brain of a living animal. Led by Northwestern University and the University of Pittsburgh, the new study offers a giant step toward understanding the brain’s millions of distinct proteins. As the building blocks of all cells including neurons, proteins hold the keys to better understanding complex brain diseases such as Parkinson’s and Alzheimer’s, which can lead to the development of new treatments. The study will be published today (August 11, 2021) in the journal Nature Communications. In the new study, researchers designed a virus to send an enzyme to a precise location in the brain of a living mouse. Derived from soybeans, the enzyme genetically tags its neighboring proteins in a predetermined location. After validating the technique by imaging the brain with fluorescence and electron microscopy, the researchers found their technique took a snapshot of the entire set of proteins (or proteome) inside living neurons, which can then be analyzed postmortem with mass spectroscopy. “Similar work has been done before in cellular cultures. But cells in a dish do not work the same way they do in a brain, and they don’t have the same proteins in the same places doing the same things,” said Northwestern’s Yevgenia Kozorovitskiy, senior author of the study. “It’s a lot more challenging to do this work in the complex tissue of a mouse brain. Now we can take that proteomics prowess and put it into more realistic neural circuits with excellent genetic traction.” By chemically tagging proteins and their neighbors, researchers can now see how proteins work within a specific, controlled area and how they work with one another in a proteome. Along with the virus carrying the soybean enzyme, the researchers also used their virus to carry a separate green fluorescent protein. “The virus essentially acts as a message that we deliver,” Kozorovitskiy said. “In this case, the message carried this special soybean enzyme. Then, in a separate message, we sent the green fluorescent protein to show us which neurons were tagged. If the neurons are green, then we know the soybean enzyme was expressed in those neurons.” Kozorovitskiy is the Soretta and Henry Shapiro Research Professor of Molecular Biology, an associate professor of neurobiology in Northwestern’s Weinberg College of Arts and Sciences and a member of the Chemistry of Life Processes Institute. She co-led the work with Matthew MacDonald, an assistant professor of psychiatry at the University of Pittsburgh Medical Center. Protein targeting plays catch-up While genetic targeting has completely transformed biology and neuroscience, protein targeting has woefully lagged behind. Researchers can amplify and sequence genes and RNA to identify their exact building blocks. Proteins, however, cannot be amplified and sequenced in the same manner. Instead, researchers have to divide proteins into peptides and then put them back together, which is a slow and imperfect process. “We have been able to gain a lot of traction with genetic and RNA sequencing, but proteins have been out of the loop,” Kozorovitskiy said. “Yet everyone recognizes the importance of proteins. Proteins are the ultimate effectors in our cells. Understanding where proteins are, how they work and how they work relative to each other is really important.” “Mass spectroscopy-based proteomics is a powerful technique,” said Vasin Dumrongprechachan, a Ph.D. candidate in Kozorovitskiy’s laboratory and the paper’s first author. “With our approach, we can start mapping the proteome of various brain circuits with high precision and specificity. We can even quantify them to see how many proteins are present in different parts of neurons and the brain.” Next step: Better understanding brain diseases Now that this new system has been validated and is ready to go, the researchers can apply it to mouse models for disease to better understand neurological illnesses. “We are hoping to extend this approach to start identifying the biochemical modifications on neuronal proteins that occur during specific patterns of brain activity or with changes induced by neuroactive drugs to facilitate clinical advances,” Dumrongprechachan said. “We look forward to taking this to models related to brain diseases and connect those studies to postmortem proteomics work in the human brain,” Kozorovitskiy said. “It’s ready to be applied to those models, and we can’t wait to get started.” Reference: “Cell-type and subcellular compartment-specific APEX2 proximity labeling reveals activity-dependent nuclear proteome dynamics in the striatum” by V. Dumrongprechachan, R. B. Salisbury, G. Soto, M. Kumar, M. L. MacDonald and Y. Kozorovitskiy, 11 August 2021, Nature Communications. DOI: 10.1038/s41467-021-25144-y The study was supported by the National Institute of Mental Health (award numbers R56MH113923, R01MH117111 and R01MH118497), National Institute of Neurological Disorders and Stroke (award number R01NS107539), National Science Foundation (CAREER 1846234), the American Heart Association (award number 19PRE34380056), the Beckman Young Investigator Award, Searle Scholar Award, Rita Allen Foundation Scholar Award and Sloan Research Fellowship. RRG455KLJIEVEWWF |
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