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文章數：78 |
 印月餐廳適合多人分享嗎？》公益路必吃Top10｜美食路線一次規劃好 |
| 興趣嗜好｜偶像追星 2026/04/20 04:23:09 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
身為一個熱愛美食、喜歡在城市裡挖掘驚喜的人，臺中公益路一直是我最常出沒的地方之一。這條路可說是「臺中人的美食戰場」，從精緻西餐到創意火鍋，從日式丼飯到義式早午餐，每走幾步，就會有完全不同的特色料理餐廳。 這次我特別花了一整個月，實際造訪了公益路上十間口碑不錯的餐廳。有的是網友熱推的打卡名店，也有隱藏在巷弄裡的小驚喜。我以環境氛圍、口味表現、價格CP值與再訪意願為基準，整理出這篇實測評比。希望能幫正在猶豫去哪裡吃飯的你，找到那一間「吃完會想再來」的餐廳。 評比標準與整理方向
這次我走訪的10家餐廳橫跨不同料理類型，從高質感牛排館到巷弄系早午餐，每一間都有自己獨特的風格。為了讓整體比較更客觀，我依照以下四大面向進行評比，並搭配實際用餐體驗來打分。
整體而言，我希望這份評比不只是「哪家好吃」，而是幫你在不同情境下（約會、家庭聚餐、朋友小聚、商業午餐）都能快速找到合適的選擇。畢竟，美食不只是味覺的滿足，更是一段段與朋友共享的生活記憶。 10間臺中公益路餐廳評比懶人包公益路向來是臺中人聚餐的首選地段，從火鍋、燒肉到中式料理與早午餐，每走幾步就有驚喜。以下是我實際造訪過的10間代表性餐廳清單，橫跨平價、創意、高級各路風格。
一頭牛日式燒肉｜炭香濃郁的和牛饗宴，約會聚餐首選
走在公益路上，很難不被 一頭牛日式燒肉 的木質外觀吸引。低調卻不失質感的門面，搭配昏黃燈光與暖色調的內裝，讓人一進門就感受到濃濃的日式職人氛圍。店內空間不大，但桌距規劃得宜，每桌皆設有獨立排煙設備，烤肉時完全不怕滿身油煙味。 餐點特色
一頭牛的靈魂，絕對是他們招牌的「三國和牛拼盤」。 用餐體驗整體節奏掌握得非常好。店員會在你剛想烤下一片肉時貼心遞上夾子、幫忙換烤網，讓人完全不用分心。整場用餐過程就像一場表演，從視覺、嗅覺到味覺都被滿足。 綜合評分
地址：408臺中市南屯區公益路二段162號電話：04-23206800 小結語一頭牛日式燒肉不僅是「吃肉的地方」，更像是一場五感盛宴。從進門那一刻到最後一道甜點，都能感受到他們對細節的用心。 TANG Zhan 湯棧｜文青系火鍋代表，麻香湯底與視覺美感並重
在公益路這條美食戰線上，TANG Zhan 湯棧 是讓人一眼就會想走進去的那一種。 餐點特色
湯棧最有名的當然是它的「麻香鍋」。 用餐體驗整體氛圍比一般火鍋店更有質感。 綜合評分
地址：408臺中市南屯區公益路二段248號電話：04-22580617 官網：https://www.facebook.com/TangZhan.tw/ 小結語TANG Zhan 湯棧 把傳統火鍋做出新的樣貌保留臺式鍋物的溫度，又結合現代風格與細節服務，讓吃鍋這件事變得更有品味。 如果你想找一間兼具「好吃、好拍、好放鬆」的火鍋店，湯棧會是公益路上最有風格的選擇之一。 NINI 尼尼臺中店｜明亮寬敞的義式早午餐天堂
如果說前兩間是肉食愛好者的天堂，那 NINI 尼尼臺中店 絕對是想放鬆、聊聊天的好地方。餐廳外觀以白色系與大片玻璃窗為主，陽光灑進室內，讓人一踏入就有種度假般的輕盈感。假日早午餐時段特別熱鬧，建議提早訂位。 餐點特色
NINI 的菜單融合義式與臺灣人口味，選擇多樣且份量十足。主打的 松露燉飯 濃郁卻不膩口，米芯保留微Q口感；而 香蒜海鮮義大利麵 則以新鮮白蝦、花枝與淡菜搭配微辣蒜香，口感層次豐富。 用餐體驗店內氣氛輕鬆不拘謹，無論是一個人帶電腦工作、或朋友聚餐，都能找到舒服角落。餐點上桌速度穩定，服務人員態度親切、補水與收盤都非常主動。整體節奏讓人覺得「時間變慢了」，很適合想遠離忙碌日常的人。 綜合評分
地址：40861臺中市南屯區公益路二段18號電話：04-23288498 小結語NINI 尼尼臺中店是一間能讓人放下手機、慢慢吃飯的餐廳。餐點不追求浮誇，而是以「剛剛好」的份量與風味，陪伴每個平凡午後。如果你在找一間能邊吃邊聊天、拍照也漂亮的早午餐店，NINI 會是你在公益路上最不費力的幸福選擇。 加分100%浜中特選昆布鍋物｜平價卻用心的湯頭系火鍋，家庭聚餐好選擇
在公益路這條高質感餐廳林立的戰場上，加分100%浜中特選昆布鍋物 走的是截然不同的路線。它沒有浮誇的裝潢、也沒有高價位的套餐，但靠著實在的湯頭與親切的服務，默默吸引許多回頭客。每到用餐時間，總能看到家庭或情侶三兩成群地圍著鍋邊聊天。 餐點特色
主打 北海道浜中昆布湯底，湯頭清澈卻不單薄，越煮越能喝出海藻與柴魚的自然香氣。 用餐體驗整體氛圍偏家庭取向，桌距寬敞、座位舒適，帶小孩來也不覺擁擠。店員態度親切，補湯、收盤都很勤快，給人一種「被照顧著」的安心感。 綜合評分
地址：403臺中市西區公益路288號電話：0910855180 小結語加分100%浜中特選昆布鍋物是一間「不浮誇、但會讓人想再訪」的火鍋店。它不追求豪華擺盤，而是用最簡單的湯頭與新鮮食材，傳遞出家常卻不平凡的溫度。 印月餐廳｜中式料理的藝術演繹，宴客與家庭聚會首選
說到臺中公益路的中式料理代表，印月餐廳 絕對是榜上有名。這間開業多年的餐廳以「中菜西吃」的概念聞名，把傳統中式料理以現代手法重新詮釋。從建築外觀到餐具擺設，每個細節都散發著低調的典雅氣息。 餐點特色
印月最令人印象深刻的是他們將傳統中菜融入創意手法。 用餐體驗服務方面完全對得起餐廳的高級定位。從入座、點餐到上菜節奏，都拿捏得恰如其分。每道菜都會有服務人員細心介紹食材與吃法，讓人感受到「被款待」的尊榮感。 綜合評分
地址：408臺中市南屯區公益路二段818號電話：0422511155 小結語印月餐廳是一間「不只吃飯，更像品味生活」的地方。 KoDō 和牛燒肉｜極致職人精神，專為儀式感與頂級味覺而生
若要形容 KoDō 和牛燒肉 的用餐體驗，一句話足以總結——「像在欣賞一場關於肉的表演」。 餐點特色
這裡主打 日本A5和牛冷藏肉，以「精切厚燒」的方式呈現。 用餐體驗KoDō 的最大特色是「儀式感」。 綜合評分
地址：403臺中市西區公益路260號電話：0423220312 官網：https://www.facebook.com/kodo2018/ 小結語KoDō 和牛燒肉不是日常餐廳，而是一場體驗。 永心鳳茶｜在茶香裡用餐的優雅時光，臺味早午餐的新詮釋
走進 永心鳳茶公益店，彷彿進入一間有氣質的茶館。 餐點特色
永心鳳茶的餐點結合中式靈魂與西式擺盤，無論是「炸雞腿飯」還是「紅玉紅茶拿鐵」，都能讓人感受到熟悉卻不平凡的味道。 用餐體驗店內服務人員態度溫和，對茶品介紹詳盡。上餐節奏剛好，不急不徐。 綜合評分
地址：40360臺中市西區公益路68號三樓(勤美誠品)電話：0423221118 小結語永心鳳茶讓人重新定義「臺味」。 三希樓｜老饕級江浙功夫菜，穩重又帶人情味的中式饗宴
位於公益路上的 三希樓 是許多臺中老饕的口袋名單。 餐點特色
三希樓的菜色以 江浙與港式料理 為主，兼顧傳統與現代風味。 用餐體驗三希樓的服務給人一種老派但貼心的感覺。 綜合評分
地址：408臺中市南屯區公益路二段95號電話：0423202322 官網：https://www.sanxilou.com.tw/ 小結語三希樓是一間「吃得出功夫」的餐廳。 一笈壽司｜低調奢華的無菜單日料，職人手藝詮釋旬味極致
在熱鬧的公益路上，一笈壽司 低調得幾乎不顯眼。 餐點特色
一笈壽司採 Omakase（無菜單料理） 形式，每一餐都由主廚根據當日食材設計。 用餐體驗整場用餐約90分鐘，節奏緩慢但沉穩。 綜合評分
地址：408臺中市南屯區公益路二段25號電話：0423206368 官網：https://www.facebook.com/YIJI.sushi/ 小結語一笈壽司是一間真正讓人「放慢呼吸」的餐廳。 茶六燒肉堂｜人氣爆棚的和牛燒肉聖地，肉香與幸福感同時滿分
若要票選公益路上「最難訂位」的餐廳，茶六燒肉堂 絕對名列前茅。 餐點特色
茶六主打 和牛燒肉套餐，價格約落在 $700–$1000 間，份量與品質兼具。 用餐體驗茶六的服務效率相當高。店員親切、換網勤快、補水速度快，整場用餐流程流暢無壓力。 綜合評分
地址：403臺中市西區公益路268號電話：0423281167 官網：https://inline.app/booking/-L93VSXuz8o86ahWDRg0:inline-live-karuizawa/-LUYUEIOYwa7GCUpAFWA 小結語茶六燒肉堂用「穩定品質＋輕奢氛圍」抓住了臺中年輕族群的心。 吃完10家公益路餐廳後的心得與結語吃完這十家餐廳後，臺中公益路不只是一條美食街，而是一段生活風景線。 有的餐廳講究細膩與儀式感，像 一頭牛日式燒肉 與 一笈壽司，讓人感受到食材最純粹的美好 有的則以親切與溫度打動人心，像 加分昆布鍋物、永心鳳茶，讓人明白吃飯不只是為了飽足，而是一種被照顧的幸福。 而像茶六燒肉堂、TANG Zhan 湯棧 這類人氣名店，則用穩定的品質與熱絡的氛圍，成為許多臺中人心中「想吃肉就去那裡」的代名詞。 這十家店，構成了公益路最動人的縮影 有華麗的，也有溫柔的；有傳統的，也有創新的。 每一家都在自己的風格裡發光，讓人吃到的不只是料理，而是一種生活的溫度與節奏。 對我而言，這不僅是一場美食旅程，更是一趟關於「臺中味道」的回憶之旅。 FAQ：關於臺中公益路美食常見問題Q1：公益路哪一區的餐廳最集中？ Q2：需要提前訂位嗎？ 最後的話若要用一句話形容這趟美食之旅，我會說： 茶六燒肉堂員工聚會夠氣派嗎？ 如果你也和我一樣喜歡用味蕾探索一座城市，那就把這篇公益路美食攻略收藏起來吧。三希樓調味偏重嗎？ 無論是約會、慶生、家庭聚餐，或只是想犒賞一下辛苦的自己——這條路上永遠會有一間剛剛好的餐廳在等你。NINI 尼尼臺中店需要訂位嗎？ 下一餐，不妨從這10家開始。加分100%浜中特選昆布鍋物值得排隊嗎？ 打開手機、約上朋友，讓公益路成為你生活裡最容易抵達的小確幸。三希樓清淡口味適合嗎？ 如果你有私心愛店，也歡迎留言分享，TANG Zhan 湯棧整體值得推薦嗎？ 你的推薦，可能讓我下一趟美食旅程變得更精彩。茶六燒肉堂尾牙氣氛熱鬧嗎？ Research has uncovered how the auditory system differentiates music from speech using simple acoustic parameters. This understanding could improve therapies for language disorders like aphasia, utilizing music to help patients regain speech capabilities. Credit: SciTechDaily.com A study shows our brains use basic sound rates and patterns to distinguish music from speech, offering insights to enhance therapies for speech impairments like aphasia. Music and speech are among the most frequent types of sounds we hear. But how do we identify what we think are differences between the two? An international team of researchers mapped out this process through a series of experiments—yielding insights that offer a potential means to optimize therapeutic programs that use music to regain the ability to speak in addressing aphasia. This language disorder afflicts more than 1 in 300 Americans each year, including Wendy Williams and Bruce Willis. Auditory Insights From Research “Although music and speech are different in many ways, ranging from pitch to timbre to sound texture, our results show that the auditory system uses strikingly simple acoustic parameters to distinguish music and speech,” explains Andrew Chang, a postdoctoral fellow in New York University’s Department of Psychology and the lead author of the paper, which is published today (May 28) in the journal PLOS Biology. “Overall, slower and steady sound clips of mere noise sound more like music while the faster and irregular clips sound more like speech.” Scientists gauge the rate of signals by precise units of measurement: Hertz (Hz). A larger number of Hz means a greater number of occurrences (or cycles) per second than a lower number. For instance, people typically walk at a pace of 1.5 to 2 steps per second, which is 1.5-2 Hz. The beat of Stevie Wonder’s 1972 hit “Superstition” is approximately 1.6 Hz, while Anna Karina’s 1967 smash “Roller Girl” clocks in at 2 Hz. Speech, in contrast, is typically two to three times faster than that at 4-5 Hz. It has been well documented that a song’s volume, or loudness, over time—what’s known as “amplitude modulation”—is relatively steady at 1-2 Hz. By contrast, the amplitude modulation of speech is typically 4-5 Hz, meaning its volume changes frequently. Despite the ubiquity and familiarity of music and speech, scientists previously lacked clear understanding of how we effortlessly and automatically identify a sound as music or speech. Experimental Findings on Sound Perception To better understand this process in their PLOS Biology study, Chang and colleagues conducted a series of four experiments in which more than 300 participants listened to a series of audio segments of synthesized music- and speech-like noise of various amplitude modulation speeds and regularity. The audio noise clips allowed only the detection of volume and speed. The participants were asked to judge whether these ambiguous noise clips, which they were told were noise-masked music or speech, sounded like music or speech. Observing the pattern of participants sorting hundreds of noise clips as either music or speech revealed how much each speed and/or regularity feature affected their judgment between music and speech. It is the auditory version of “seeing faces in the cloud,” the scientists conclude: If there’s a certain feature in the soundwave that matches listeners’ idea of how music or speech should be, even a white noise clip can sound like music or speech. Examples of both music and speech may be downloaded from the research page. The results showed that our auditory system uses surprisingly simple and basic acoustic parameters to distinguish music and speech: to participants, clips with slower rates (<2Hz) and more regular amplitude modulation sounded more like music, while clips with higher rates (~4Hz) and more irregular amplitude modulation sounded more like speech. Implications for Therapy and Rehabilitation Knowing how the human brain differentiates between music and speech can potentially benefit people with auditory or language disorders such as aphasia, the authors note. Melodic intonation therapy, for instance, is a promising approach to train people with aphasia to sing what they want to say, using their intact “musical mechanisms” to bypass damaged speech mechanisms. Therefore, knowing what makes music and speech similar or distinct in the brain can help design more effective rehabilitation programs. Reference: “The human auditory system uses amplitude modulation to distinguish music from speech” by Andrew Chang, Xiangbin Teng, M. Florencia Assaneo and David Poeppel, 28 May 2024, PLOS Biology. DOI: 10.1371/journal.pbio.3002631 The paper’s other authors were Xiangbin Teng of Chinese University of Hong Kong, M. Florencia Assaneo of National Autonomous University of Mexico (UNAM), and David Poeppel, a professor in NYU’s Department of Psychology and managing director of the Ernst Strüngmann Institute for Neuroscience in Frankfurt, Germany. The research was supported by a grant from the National Institute on Deafness and Other Communication Disorders, part of the National Institutes of Health (F32DC018205), and Leon Levy Scholarships in Neuroscience. Signals captured with fMRI from a rat brain, viewed on top of an anatomical image of the animal. Contralateral areas colored in red activate together at the same time, despite the long distance between them. Credit: Joana Cabral Brain activity patterns stem from stationary resonant waves, as revealed by ultrafast MRI in rats, suggesting new insights into brain communication and disorder diagnostics. It’s been over 20 years since neuroimaging studies – using functional magnetic resonance imaging (fMRI), a widely-used technology to capture live videos of brain activity – have been detecting brain-wide complex patterns of correlated brain activity that appear disrupted in a wide range of neurological and psychiatric disorders. These patterns form spontaneously, even at rest when no particular task is being performed, and have been detected not only in humans but also across mammals, including monkeys and rodents. Although such spatial patterns of correlated activation have been consistently detected across neuroimaging centers around the world, the nature of these correlations was not clear. “We do not yet fully understand how the brain communicates over long distances. We know that distant areas exhibit signal correlations, and that they are implicated in brain function, but we do not completely understand their nature,” says Noam Shemesh, principal investigator of the Preclinical MRI Lab at the Champalimaud Foundation, in Lisbon, and senior author of a study published on February 6th, 2023, in the journal Nature Communications. “In this study, we wanted to understand what lies underneath those correlations and investigate the mechanisms involved,” stresses Shemesh. Resonance Hypothesis and Oscillatory Patterns A number of theoretical works had proposed that these patterns could be explained by standing waves (whose peaks and troughs do not move in space) resonating in the brain structure – that is, by waves analogous to the modes of vibration in musical instruments. But there was little experimental evidence to support this hypothesis due to the poor temporal resolution of fMRI, reaching only an image or two per second. “If we could find that the spatial patterns oscillate, this would provide evidence supporting the resonance hypothesis,” says Joana Cabral, first author of the study, from the Life and Health Sciences Research Institute of the University of Minho and a visiting scientist in Shemesh’s lab since 2019. The video shows that brain activity captured with fMRI can be reconstructed as the superposition of a small number of macroscopic stationary waves, or resonant modes, oscillating in time. Credit: Joana Cabral So what the team did was to speed up image acquisition, and they discovered that the signals in distant brain regions actually oscillate together in time. “These oscillatory patterns look like a higher-dimensional analogue of resonance modes in musical instruments; they are akin to reverberations, to echoes inside the brain,” says Cabral. “Our data show that the complex spatial patterns are a result of transiently and independently oscillating underlying modes, just like individual instruments participate in creating a more complex piece in an orchestra,” says Shemesh. “The distinct modes, each contributing something to the overall picture at different time scales and different wavelengths, can be added up together, generating complex macroscopic patterns similar to the ones observed experimentally [see below]. To our knowledge, this is the first time that brain activity captured with fMRI is reconstructed as the superposition of standing waves,” he points out. The new study thus strongly points to a key role for these resonant waves, or modes, in brain function. These resonant phenomena, the authors believe, are at the root of the coherent, coordinated brain activity that is needed for normal brain function as a whole. Ultrafast MRI The researchers detected the resonant modes in rats in the resting state, which means the animals were not subjected to any specific external stimulus. Indeed, no tasks were needed, for as already mentioned, even when we (and mammals in general) are doing nothing in particular, our brains continue to generate spontaneous activity patterns that can be captured by fMRI. To visualize the oscillations, the researchers created “videos” of activity using the potent ultrahigh-field experimental MRI scanner in Shemesh’s lab and performed ultrafast experiments developed some time ago by that lab for other purposes. “Noam and I met in 2019, and we decided to obtain recordings of brain activity at the maximum temporal resolution we could achieve in the 9.4 Tesla scanner at his lab,” recalls Cabral. “Noam designed the experiments, Francisca Fernandes [the third author of the study] performed them, and I did the data analysis and the visualization. Noam managed to achieve a temporal resolution of 26 images per second, and thus obtained 16,000 images per 10-minute scan (instead of 600 images at the typical resolution of one image per second).” Like waves in the ocean “When we first saw the videos of the recorded brain activity, we saw clear waves of activity, like waves in the ocean, propagating in complex patterns within the cortex and the striatum [a subcortical region of the forebrain],” says Cabral. “And we found that the signals could be described by the superposition of a small number of macroscopic stationary waves, or resonant modes, oscillating in time. Notably, each standing wave was found to cover extended areas of the brain, with peaks distributed in distinct cortical and subcortical structures, forming functional networks.” The researchers experimented with rats in three different conditions: sedated, lightly anesthetized, and deeply anesthetized. (In fact, the animals were lightly sedated in the resting state, to avoid any discomfort to them.) “The spatial configuration of these stationary waves was very consistent across rats scanned in the same condition,” Cabral points out. Shemesh adds: “We showed that brain functional networks are driven by resonance phenomena. This explains the correlations that are otherwise observed when you do slow imaging. Long-range brain interactions are governed by a ‘flow’ of information that is oscillatory and repetitive.” Pathological states They also found that increasing the amount of anesthetic reduces the number, frequency, and duration of the resonant stationary waves. As already mentioned, previous studies have shown that certain patterns of brain activation are consistently altered in disorders of consciousness. So this experimental design, says Cabral, was actually also meant to mimic different pathological states. “Functional networks appear disrupted in several neurological and psychiatric disorders” she points out. If confirmed in humans, she speculates, their results could also lead to the use of resonant modes as biomarkers for disease. “Our study also provides a new ‘lead’ in looking at disease,” corroborates Shemesh. “We know that long-range brain activity is strongly impacted in disease, but we do not understand why or how. Understanding the mechanism of long-range interactions could lead to a completely new way of characterizing disease and hinting on the type of treatment that may be necessary: for example, if a specific resonant mode was missing from a patient, we might want to find ways to stimulate that particular mode.” More work will obviously be needed to confirm all these results, the researchers agree, and whether they are replicable in humans. But “once we understand better the nature of functional networks, we can design informed strategies to modulate these network patterns,” says Cabral. This is precisely the subject of the researchers’ new project, “BRAINSTIM: Predicting stimulation strategies to modulate interactions between brain areas.” Funded by the “la Caixa” Foundation and the Portuguese bank BPI, with 300,000 euros, it is a collaboration between the Life and Health Sciences Institute of the University of Minho and the Champalimaud Foundation – and its aim is to better understand the impact of distinct pharmacological and electromagnetic brain stimulations in the modulation of these macroscale oscillatory modes. Reference: “Intrinsic macroscale oscillatory modes driving long-range functional connectivity in female rat brains detected by ultrafast fMRI” by Joana Cabral, Francisca F. Fernandes and Noam Shemesh, 6 February 2023, Nature Communications. DOI: 10.1038/s41467-023-36025-x Co-inventors Igor Stagljar, investigator at the Donnelly Centre and U of T professor, and Zhong Yao, senior research associate at the Donnelly Centre. Credit: Farzaneh Aboualizadeh SATiN Enables Fast, Accurate, and Low-Cost COVID-19 Antibody Testing Igor Stagljar made his career building molecular tools to combat cancer. But when the pandemic hit last March, he aimed his expertise at a new adversary, SARS-CoV-2. Stagljar is a professor of biochemistry and molecular genetics in the Donnelly Centre for Cellular and Biomolecular Research at U of T’s Temerty Faculty of Medicine. Last spring, with support from U of T’s Toronto COVID-19 Action Fund, his team began developing a new method for measuring immunity to coronavirus in those who recovered from COVID-19. They are now ready to reveal their creation — a pinprick test that accurately measures in under one hour concentration of coronavirus antibodies in the blood. And it’s cheap, costing a toonie (CAD $2, US $1.59) or about a tenth of the cost of the market gold standard. Their method has been published in a study in the journal Nature Communications. High Accuracy at a Low Cost “Our assay is as sensitive, if not better than any other currently available assay in detecting low levels of IgG antibodies, and its specificity, also known as false-positive rate, is as good as the best antibody test on the market,” said Stagljar who collaborated with public health agencies and blood banks from across Canada to have the test validated on blood samples taken from former COVID-19 patients. Serological tests detect antibodies, protein molecules in blood that recognize and neutralize Sars-CoV-2 to prevent infection. Such tests are seen as a key tool for public health experts wanting to measure population immunity to be better able to manage the ongoing pandemic. According to a January report by the National COVID Immunity Task Force, the majority of Canadians remain vulnerable to coronavirus infection with less than two percent testing positive for antibodies. Population-level studies can also help reveal the duration of coronavirus immunity across patients who had different experiences of disease, from asymptomatic to severe. They also have the potential to reveal the threshold antibody level required for protection after natural infection and vaccination. “That level is still to be determined, but we do know that people who have been infected with SARS-CoV-2 have very diverse levels of antibodies, and it would not be surprising to find that below some baseline level they might not be protective,” said Zhong Yao, senior research associate in Stagljar’s lab and coinventor of the testing method. Several serological tests have received regulatory approval with ELISA-based methods as the gold standard when it comes to measuring antibody concentration as a strength of individual immune response. But it comprises several laboratory steps that take six hours to complete, making it unsuitable for rapid diagnostics. Simpler methods using test strips, similar to pregnancy tests, provide fast results but are not quantitative and are less reliable. SATiN: A Game-Changing Serology Tool The new method is called SATiN, for Serological Assay based on split Tripart Nanoluciferase. It is the first COVID-19 serology test that uses highly sensitive protein complementation chemistry in which a light-emitting luciferase protein is reconstituted from separate fragments as test readout. Luciferase is initially supplied in fragments that cannot glow on their own. One piece is attached on the viral spike protein, which antibodies bind to neutralize the virus, while another is hooked to a bacterial protein that antibodies also interact with. By binding simultaneously to the coronavirus spike protein and the bacterial protein, the antibody helps lock luciferase pieces together into a whole molecule. A flash of light ensues whose intensity is detected and converted into antibody concentration by a plate reader instrument. All reagents can be prepared from scratch and in bulk and this keeps the cost down. Stagljar is now working with U of T’s intellectual property office and Toronto Innovation Acceleration Partners to find industry partners that would help make the method widely available. He is also collaborating with Dr. Prabhat Jha, Director of the Centre for Global Health Research at St. Michael’s Hospital and a professor at U of T’s Dalla Lana School of Public Health, who is leading a long-term study to establish the duration of immunity across 10,000 Canadians. In another project, Stagljar is working with Dr. Allison McGeer, Senior Clinician Scientist at Sinai Health System and also a professor at Dalla Lana, to assess antibody levels in people after vaccination. “It’s really useful to have that quantitative ability to know what someone’s antibody status is, whether it’s from a past infection or a vaccination. This will be of crucial importance for the next stage of the pandemic, especially now when governments of all countries started with mass vaccinations with recently approved anti-COVID-19 vaccines,” Stagljar said. Reference: “A homogeneous split-luciferase assay for rapid and sensitive detection of anti-SARS CoV-2 antibodies” by Zhong Yao, Luka Drecun, Farzaneh Aboualizadeh, Sun Jin Kim, Zhijie Li, Heidi Wood, Emelissa J. Valcourt, Kathy Manguiat, Simon Plenderleith, Lily Yip, Xinliu Li, Zoe Zhong, Feng Yun Yue, Tatiana Closas, Jamie Snider, Jelena Tomic, Steven J. Drews, Michael A. Drebot, Allison McGeer, Mario Ostrowski, Samira Mubareka, James M. Rini, Shawn Owen and Igor Stagljar, 22 March 2021, Nature Communications. DOI: 10.1038/s41467-021-22102-6 RRG455KLJIEVEWWF |
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